Satellite NG2 progenitor cells share common glutamatergic inputs with associated interneurons in the mouse dentate gyrus.
Satellite NG2 progenitor cells share common glutamatergic inputs with associated interneurons in the mouse dentate gyrus.
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DOI：
10.1523/jneurosci.1355-08.2008
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发表时间：
2008-07-23
期刊：
影响因子：
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通讯作者：
Gallo V
中科院分区：
文献类型：
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作者：
Mangin JM;Kunze A;Chittajallu R;Gallo V
一些研究已经提供了证据，表明表达NG2 （NG2+）的祖细胞在解剖学上与灰质区域的神经元相关。通过分析小鼠齿状回门部NG2+细胞的空间分布，我们证实NG2+细胞确实与中间神经元密切相关。为了确定这种解剖上的接近是否反映了特定的生理相互作用，我们在出生后3至21天对肺门NG2+细胞和中间神经元进行了膜片钳记录。我们首先观察到肝门NG2+细胞表现出自发性兴奋性突触电流（sEPSC），其频率和振幅在出生后的前三周增加。与此同时，sEPSCs的上升时间和衰减时间显著减少，这表明NG2+细胞中的谷氨酸能突触经历了一个成熟过程，这让人想起了同一时期在神经元中报道的成熟过程。我们还观察到门叶中间神经元和相关的NG2+细胞同样整合到局部网络中，接受来自颗粒细胞和CA3锥体神经元的兴奋输入。通过对记录，我们发现GABAergic拮抗剂诱导的活动爆发在两种细胞类型之间是强烈同步的，并且这些爆发的幅度是正相关的。最后，通过应用碳醇来增加EPSC活性，我们观察到，与相隔超过200微米的细胞相比，紧密接触的细胞更有可能表现出同步的EPSC。NG2+细胞在密切相关的神经元中感知活动模式，这一发现表明NG2+细胞的功能受到局部网络的精细调节。
Several studies have provided evidence that NG2-expressing (NG2+) progenitor cells are anatomically associated to neurons in gray matter areas. By analyzing the spatial distribution of NG2+ cells in the hilus of the mouse dentate gyrus, we demonstrate that NG2+ cells are indeed closely associated to interneurons. In order to define whether this anatomical proximity reflected a specific physiological interaction, we performed patch-clamp recordings on hilar NG2+ cells and interneurons between 3 and 21 postnatal days. We first observed that hilar NG2+ cells exhibit spontaneous excitatory synaptic currents (sEPSC) whose frequency and amplitude increase during the first three postnatal weeks. At the same time, the rise-time and decay-time of sEPSCs significantly decreased, suggesting that glutamatergic synapses in NG2+ cells undergo a maturation process that is reminiscent of what has been reported in neurons during the same time period. We also observed that hilar interneurons and associated NG2+ cells are similarly integrated into the local network, receiving excitatory inputs from both granule cells and CA3 pyramidal neurons. By performing pair-recordings, we found that bursts of activity induced by GABAergic antagonists were strongly synchronized between both cell types and that the amplitude of these bursts was positively correlated. Finally, by applying carbachol to increase EPSC activity, we observed that closely apposed cells were more likely to exhibit synchronized EPSCs than cells separated by more than 200 microns. The finding that NG2+ cells are sensing patterns of activity arising in closely associated neurons suggests that NG2+ cell function is finely regulated by the local network.