Comparative study of the epidemiology of rotavirus in children from a community-based birth cohort and a hospital in South India
Comparative study of the epidemiology of rotavirus in children from a community-based birth cohort and a hospital in South India
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DOI：
10.1128/jcm.01882-05
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发表时间：
2006-07-01
影响因子：
9.4
通讯作者：
Kang, Gagandeep
中科院分区：
文献类型：
--
作者：
Banerjee, Indrani;Ramani, Sasirekha;Kang, Gagandeep
轮状病毒胃肠炎是全球儿童严重脱水性腹泻的主要原因。本研究比较了351名社区儿童和343名同期住院儿童的轮状病毒腹泻。在腹泻发作期间获得临床信息和粪便标本。粪便标本进行VP 6抗原筛查，阳性标本经逆转录-PCR进行G、P分型。轮状病毒在82/1，152（7.1%）的社区腹泻和94/343（27.4%）的医院病例中检出。社区中受影响儿童的中位年龄（7.5对10.5个月）和症状的平均严重程度（Vesikari评分，7.6 +/- 3.4对11 +/- 2.5）较低。社区中母乳喂养的儿童比例高于住院儿童（73%对34.8%）。在社区中，在有症状的患者中确定的基因型，按频率顺序为G1（36.5%），G10（17.1%），G2（15.9%）和G9（7.3%）和混合感染（7.3%）。最常见的G-P组合是GIP[8]、G2 P [4]、GIP[4]和G10 P [11]。住院患儿G型分布依次为G1（46.8%）、G9（19.1%）、G2（8.5%）、G10（1.1%）和混合感染（4.3%）。最常见的G-P组合是GIP[81]和G9 P [8]。本研究记录了社区和医院中轮状病毒的显著遗传异质性。G10 P [11]菌株类似于疫苗候选菌株，在社区中引起疾病，表明需要对候选疫苗进行仔细的流行病学研究和安全性研究。
Rotavirus gastroenteritis is the major cause of severe dehydrating diarrhea in children worldwide. This study compares rotavirus diarrhea in 351 children in a community-based cohort and 343 children admitted to a hospital during the same period. Clinical information and fecal specimens were obtained during diarrheal episodes. Fecal samples were screened for VP6 antigen, and the positive samples were G and P typed by reverse transcription-PCR. Rotavirus was detected in 82/1,152 (7.1%) episodes of diarrhea in the community and 94/343 (27.4%) cases in the hospital. The median age of affected children (7.5 versus 10.5 months) and the mean severity of symptoms (Vesikari score, 7.6 +/- 3.4 versus 11 +/- 2.5) were lower in the community. A larger proportion of children in the community were breast-fed than were children admitted to the hospital (73% versus 34.8%). In the community, the genotypes identified in symptomatic patients, in order of frequency, were G1 (36.5%), G10 (17.1%), G2 (15.9%), and G9 (7.3%) and mixed infections (7.3%). The most common G-P combinations were GIP[8], G2P[4], GIP[4], and G10P[11]. The distribution of G types from hospitalized children was G1 (46.8%), G9 (19.1%), G2 (8.5%), G10 (1.1%), and 4.3% mixed infections. The most common G-P combinations were GIP[81 and G9P[8]. This study documents significant genetic heterogeneity of rotaviruses in the community and the hospital. G10P[11] strains resembling a vaccine candidate strain caused disease in the community, indicating the need for careful epidemiological studies as well as safety studies for the vaccine candidates.