Comparative Genetics of the Poly-Q Tract of Ataxin-1 and Its Binding Protein PQBP-1
Comparative Genetics of the Poly-Q Tract of Ataxin-1 and Its Binding Protein PQBP-1
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Ataxin-1及其结合蛋白PQBP-1的Poly-Q束的比较遗传学
DOI：
10.1007/s10528-011-9473-1
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发表时间：
2012
期刊：
影响因子：
2.4
通讯作者：
Ueda S
中科院分区：
文献类型：
--
作者：
Kurosaki T;Gojobori J;Ueda S
已知人类PQBP-1通过其多聚谷氨酰胺（多q）束与三重重复疾病基因产物如ataxin和huntingtin相互作用。在不同的物种中，多q区在重复序列的数量和结构上都表现出广泛的差异。表面等离子体共振分析表明，人类PQBP-1与旧大陆猴ataxin-1多聚q区代表Q11之间存在明显的相互作用。使用代表新世界猴ataxin-1多q通道的Q2PQ2P4Q2未观察到应答。这表明人类PQBP-1与ataxin-1的相互作用仅限于人类和密切相关的物种。人类和小鼠PQBP-1序列的比较显示，PQBP-1的极性氨基酸丰富区域的氨基酸取代率升高，该区域负责与多聚q区结合。这可能有利于人类PQBP-1通过多q束发挥新的生物学功能。
Human PQBP-1 is known to interact with triplet repeat disease gene products such as ataxin and huntingtin through their poly-glutamine (poly-Q) tracts. The poly-Q tracts show extensive variation in both the number and the configuration of repeats among species. A surface plasmon resonance assay showed clear interaction between human PQBP-1 and Q11, representative of the poly-Q tract of the ataxin-1 of Old World monkeys. No response was observed using Q2PQ2P4Q2, representative of the poly-Q tract of the ataxin-1 of New World monkeys. This implies that the interaction of human PQBP-1 with ataxin-1 is limited to humans and closely related species. Comparison of the human and mouse PQBP-1 sequences showed an elevated amino acid substitution rate in the polar amino acid-rich domain of PQBP-1 that is responsible for binding to poly-Q tracts. This could have been advantageous to the new biological function of human PQBP-1 through poly-Q tracts.