Optimization of the separation of NDA-derivatized methylarginines by capillary and microchip electrophoresis.
Optimization of the separation of NDA-derivatized methylarginines by capillary and microchip electrophoresis.
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DOI：
10.1177/2211068211424551
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Lunte SM
中科院分区：
文献类型：
--
作者：
Linz TH;Snyder CM;Lunte SM
甲基化精氨酸 (MA)、单甲基精氨酸 (MMA)、不对称二甲基精氨酸 (ADMA) 和对称二甲基精氨酸 (SDMA) 已被证明是心血管疾病的独立预测因子。本文介绍了一种能够使用毛细管电泳 (CE) 和微芯片电泳 (MCE) 以及激光诱导荧光 (LIF) 检测快速分析 MA 的分析方法的开发进展。优化了缓冲液成分和分离电压等多个参数，以实现理想的分离。感兴趣的分析物用萘-2,3-二甲醛 (NDA) 衍生化，产生荧光 1-氰基苯并[f]异吲哚 (CBI) 衍生物，然后进行 CE 分析。 SDMA、ADMA、MMA 和精氨酸的基线分辨率在不到 8 分钟内实现。 SDMA、ADMA、MMA 和精氨酸的检测限分别确定为 15、20、25 和 5 nM，远低于预期血浆浓度。然后将 CE 分离方法转移到带有 LIF 检测的玻璃 MCE 装置。使用 14 cm 分离通道在 3 分钟内即可在芯片上解析 MA 的基线，每个物种的检测限约为 10 nM。据作者所知，这是 MCE 分离 MA 的第一份报告。
The methylated arginines (MAs) monomethylarginine (MMA), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) have been shown to be independent predictors of cardiovascular disease. This article describes progress regarding the development of an analytical method capable of rapidly analyzing MAs using capillary electrophoresis (CE) and microchip electrophoresis (MCE) with laser-induced fluorescence (LIF) detection. Several parameters including buffer composition and separation voltage were optimized to achieve an ideal separation. The analytes of interest were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) to produce fluorescent 1-cyanobenz[f]isoindole (CBI) derivatives and then subjected to CE analysis. Baseline resolution of SDMA, ADMA, MMA, and arginine was achieved in less than 8 min. The limits of detection for SDMA, ADMA, MMA, and arginine were determined to be 15, 20, 25, and 5 nM, respectively, which are well below the expected plasma concentrations. The CE separation method was then transferred to a glass MCE device with LIF detection. MAs were baseline resolved in 3 min on-chip using a 14 cm separation channel with detection limits of approximately 10 nM for each species. To the best of the authors’ knowledge, this is the first report of the separation of MAs by MCE.
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影响因子：
56.9
作者：
CULOTTA, E;KOSHLAND, DE
通讯作者：
KOSHLAND, DE
影响因子：
5.4
作者：
Ajtay, Zeno;Scalera, Fortunato;Bode-Boeger, Stefanie M.
通讯作者：
Bode-Boeger, Stefanie M.
影响因子：
19.6
作者：
Fleck, C;Janz, A;Stein, G
通讯作者：
Stein, G
影响因子：
8.3
作者：
LEE, L;WEBB, RC
通讯作者：
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影响因子：
3.1
作者：
Trapp, G;Sydow, K;Zare, RN
通讯作者：
Zare, RN