Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds
Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds
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与1-乙基-3-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺和京尼平交联的电纺聚己内酯/胶原纳米纤维促进内皮细胞再生，可能是血管支架的有前途的候选者
DOI：
10.2147/ijn.s192699
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发表时间：
2019-01-01
影响因子：
8
通讯作者：
Zhang, Haibo
中科院分区：
文献类型：
--
作者：
Chen, Dian;Zhu, Tonghe;Zhang, Haibo
目的：一个有前途的血管支架必须具有令人满意的力学性能、良好的血液相容性和良好的组织再生能力。将天然材料与合成材料相结合是创建/增强此类支架的流行方法。然而，额外的改性对材料的影响还需要进一步探索。材料和方法：我们选择了聚己内酯（PCL），它具有优异的机械性能和生物相容性，可以与胶原蛋白结合。使用 PCL/胶原蛋白溶液创建的电纺纤维用于形成混合纳米纤维，而 PCL 和胶原蛋白的单独注射器用于创建分离的纳米纤维，从而产生不同的孔径。将混合和分离的纳米纤维与戊二醛（GA）、1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）和京尼平进行交联；因此，我们将它们命名为混合GA、混合EDC（ME）、混合京尼平（MG）、分离GA、分离EDC（SE）和分离京尼平（SG）。 结果：傅里叶变换红外（FTIR）光谱和X射线衍射表明，交联并未影响所有组中纤维的主要官能团。 ME、MG、SE 和 SG 满足所需的机械性能，并且它们还可以抵抗胶原酶降解。在血液相容性测定中，只有 ME 和 MG 表现出理想的安全性。此外，ME 和 MG 表现出最大的细胞相容性。对于血管支架来说，快速内皮化有助于防止血栓形成。根据人脐静脉内皮细胞在不同纳米纤维上的迁移情况，ME和MG也成功地促进了细胞迁移。结论：ME和MG可能是血管组织工程的有希望的候选者。研究表明，通过 EDC/N-羟基琥珀酰亚胺或京尼平交联的胶原蛋白可促进内皮细胞再生，这对血管支架的组织工程有很大好处。
Purpose: A promising vascular scaffold must possess satisfying mechanical properties, great hemocompatibility, and favorable tissue regeneration. Combining natural with synthetic materials is a popular method of creating/enhancing such scaffolds. However, the effect of additional modification on the materials requires further exploration.Materials and methods: We selected polycaprolactone (PCL), which has excellent mechanical properties and biocompatibility and can be combined with collagen. Electrospun fibers created using a PCL/collagen solution were used to fashion mixed nanofibers, while separate syringes of PCL and collagen were used to create separated nanofibers, resulting in different pore sizes. Mixed and separated nanofibers were cross-linked with glutaraldehyde (GA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and genipin; hence, we named them as mixed GA, mixed EDC (ME), mixed genipin (MG), separated GA, separated EDC (SE), and separated genipin (SG).Results: Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction showed that cross-linking did not affect the main functional groups of fibers in all groups. ME, MG, SE, and SG met the requisite mechanical properties, and they also resisted collagenase degradation. In hemocompatibility assays, only ME and MG demonstrated ideal safety. Furthermore, ME and MG presented the greatest cytocompatibility. For vascular scaffolds, rapid endothelialization helps to prevent thrombosis. According to human umbilical vein endothelial cell migration on different nanofibers, ME and MG are also successful in promoting cell migration.Conclusion: ME and MG may be promising candidates for vascular tissue engineering. The study suggests that collagen cross-linked by EDC/N-hydroxysuccinimide or genipin facilitates endothelial cell regeneration, which could be of great benefit in tissue engineering of vascular scaffolds.