Fourier transform infrared study of the halorhodopsin chloride pump.
Fourier transform infrared study of the halorhodopsin chloride pump.
复制标题
氯化盐视紫红质泵的傅里叶变换红外研究。
DOI：
10.1021/bi00407a026
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Spudich,JL
中科院分区：
文献类型：
--
作者：
Rothschild,KJ;Bousché,O;Braiman,MS;Hasselbacher,CA;Spudich,JL
修订稿于 1988 年 1 月 19 日收到 摘要：Halorhodopsin (hR) 是一种光驱动的氯泵，位于 Halo-bacterial halobium 的细胞膜中。傅里叶变换红外差分光谱已被用于研究 hR 光循环过程中发生的结构变化。亚视黄基发色团的峰频率与相关蛋白质细菌视紫红质 (bR) 光谱中观察到的峰频率相似，表明在 hR 中和 bR 中一样，在早期浴产物的形成过程中发生了全反式 13-ds 异构化。 bR 和 hR 光循环由于蛋白质结构改变而产生的光谱特征也相似。例如，hR和bR的红移初级光产物的形成导致在1730-1745-cm" 1区域中出现相似的羧基峰。然而，与bR相反，在hR光循环的后续步骤中在羧基区域中没有观察到进一步的变化，这表明额外的羧基不直接参与氯离子易位。总体而言，hR和bR光产物差异光谱中的振动的密切相似性支持了一些这两种蛋白质的能量转导和主动运输的分子机制中存在共同点。盐视紫红质 (hR) 1 是一种在盐杆菌质膜中发现的光驱动氯离子泵（Lanyi，1986），在许多方面，hR 类似于在同一生物体的紫色膜中发现的光驱动质子泵细菌视紫红质 (bR)。
Revised Manuscript Received January 19, 1988 abstract: Halorhodopsin (hR) is a light-driven chloride pump located in the cell membrane of Halo-bacterium halobium. Fourier transform infrared difference spectroscopy has been used to study structural alterations occurring during the hR photocycle. The frequencies of peaks attributed to the retinylidene chromophore are similar to those observed in the spectra of the related protein bacteriorhodopsin (bR), indicating that in hR as in bR an all-trans—13-ds isomerization occurs during formation of the early bathoproduct. Spectral featuresdue to protein structural alterations are also similar for the bR and hR photocycles. For example, formation of the red-shifted primary photoproducts of both hR and bR results in similar carboxyl peaks in the 1730-1745-cm" 1 region. However, in contrastto bR, no further changes are observed in the carboxyl region during subsequentsteps in the hR photocycle, indicating that additional carboxyl groups are not directly involved in chloride translocation. Overall, the close similarity of vibrations in hR and bR photoproduct difference spectra supports the existence of some common elements in the molecular mechanisms of energy transduction and active transport by these two proteins.Halorhodopsin (hR) 1 is a light-driven chloride pump found in the plasma membrane of Halobacterium halobium (Lanyi, 1986). In many respects, hR resembles the light-driven proton pump bacteriorhodopsin (bR) found in the purple membrane of the same organism. The two proteins have similar ab-