Evidence for mRNA expression of vascular endothelial growth factor by X-ray irradiation in a lung squamous carcinoma cell line
Evidence for mRNA expression of vascular endothelial growth factor by X-ray irradiation in a lung squamous carcinoma cell line
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DOI：
10.1016/s0304-3835(98)00160-8
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发表时间：
1998-10-23
期刊：
影响因子：
9.7
通讯作者：
Kuriyama, T
中科院分区：
文献类型：
--
作者：
Ando, S;Nojima, K;Kuriyama, T
血管内皮生长因子（VEGF）是一种多能性细胞因子，在多种血管生成条件以及某些肿瘤行为中起重要作用。本文研究了X射线照射对肺鳞癌细胞系（RERF-LC-AI）VEGF mRNA表达的诱导作用。15戈伊X射线照射后16-24 h，细胞VEGF mRNA表达明显增加，为对照组的2.5倍。用染料木素（Src酪氨酸激酶抑制剂）或H7（蛋白激酶C抑制剂）处理细胞可完全阻断X射线照射对VEGF mRNA的诱导。提示诱导机制可能与启动细胞表面Src酪氨酸激酶途径和蛋白激酶C途径有关。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Vascular endothelial growth factor (VEGF) is a multipotent cytokine which plays an important role in various angiogenic conditions as well as in some tumor behaviors. Here we examined the induction of VEGF mRNA by X-ray irradiation in a lung squamous cell carcinoma cell line (RERF-LC-AI), Irradiating the cells with 15 Gy X-rays significantly increased the mRNA expression up to 2.5-fold of control at a post-irradiation time of 16-24 h. The induction of VEGF mRNA by X-ray irradiation was completely blocked by treating cells with either genistein (Src tyrosine kinase inhibitor) or H7 (protein kinase C inhibitor). This suggests that the mechanism of induction might be concerned with the pathway which triggers Src tyrosine kinase of the cell surface and the protein kinase C pathway. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.