QUANTITATION OF MALARIA SPOROZOITES IN THE SALIVARY-GLANDS OF WILD AFROTROPICAL ANOPHELES
QUANTITATION OF MALARIA SPOROZOITES IN THE SALIVARY-GLANDS OF WILD AFROTROPICAL ANOPHELES
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DOI：
10.1111/j.1365-2915.1991.tb00522.x
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发表时间：
1991-01-01
影响因子：
1.9
通讯作者：
ROBERTS, CR
中科院分区：
文献类型：
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作者：
BEIER, JC;ONYANGO, FK;ROBERTS, CR
用显微镜对肯尼亚西部1137只自然感染的野生按蚊唾液腺中的疟原虫子孢子数进行了测定。 感染性冈比亚按蚊Giles sensu lato（n = 874）含有962个子孢子的几何平均数（GM），而不死按蚊Giles（n = 263）含有812个。 不同种属、采集技术或地点之间的子孢子几何平均数无显著差异。在感染性冈比亚按蚊中，1.7%（15/874）含有超过41，830个子孢子，最多见于不死按蚊。发现显微镜技术比酶联免疫吸附试验（ELISA）更敏感，可用于检测唾液腺中的低级子孢子感染。 1137例唾液腺感染中83.6%的唾液腺子孢子经ELISA鉴定为恶性疟原虫Welch（n = 910）、卵形疟原虫Stephens（n = 7）、三日疟原虫Grassi & Feletti（n = 3）或混合型（n = 30）。 187个腺体感染，不能确定由ELISA含有显着较少的子孢子（GM = 242）比那些可以确定（GM = 1200）。
The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites.Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus.Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).