Chemical magnetoreception: bird cryptochrome 1a is excited by blue light and forms long-lived radical-pairs.
Chemical magnetoreception: bird cryptochrome 1a is excited by blue light and forms long-lived radical-pairs.
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DOI：
10.1371/journal.pone.0001106
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发表时间：
2007-10-31
期刊：
影响因子：
3.7
通讯作者：
Mouritsen, Henrik
中科院分区：
文献类型：
--
作者：
Liedvogel, Miriam;Maeda, Kiminori;Henbest, Kevin;Schleicher, Erik;Simon, Thomas;Timmel, Christiane R.;Hore, P. J.;Mouritsen, Henrik
隐花色素（Cry）被认为是鸟类依赖光的磁罗盘定位的基础。然而，要发挥磁罗盘传感器的作用，候鸟的隐花色素必须具有一些关键的生物物理特性。最重要的是，蓝光的吸收必须产生寿命超过1微秒的自由基对。利用杆状病毒/Sf 9细胞表达系统，重组表达并纯化了园莺隐花色素1a（gwCry 1a）和Cry 1光解酶同源区（gwCry 1-PHR）。瞬态吸收测量表明，这些黄素蛋白确实是激发光在蓝色光谱范围内导致形成的自由基与毫秒寿命。这些生物物理特性表明，gwCry 1a非常适合作为初级光介导的、基于自由基对的磁罗盘受体。
Cryptochromes (Cry) have been suggested to form the basis of light-dependent magnetic compass orientation in birds. However, to function as magnetic compass sensors, the cryptochromes of migratory birds must possess a number of key biophysical characteristics. Most importantly, absorption of blue light must produce radical pairs with lifetimes longer than about a microsecond. Cryptochrome 1a (gwCry1a) and the photolyase-homology-region of Cry1 (gwCry1-PHR) from the migratory garden warbler were recombinantly expressed and purified from a baculovirus/Sf9 cell expression system. Transient absorption measurements show that these flavoproteins are indeed excited by light in the blue spectral range leading to the formation of radicals with millisecond lifetimes. These biophysical characteristics suggest that gwCry1a is ideally suited as a primary light-mediated, radical-pair-based magnetic compass receptor.
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影响因子：
--
作者：
Möller, A;Sagasser, S;Schierwater, B
通讯作者：
Schierwater, B
影响因子：
56.9
作者：
PARK, HW;KIM, ST;DEISENHOFER, J
通讯作者：
DEISENHOFER, J
影响因子：
4.8
作者：
Berndt, Alex;Kottke, Tilman;Wolf, Eva
通讯作者：
Wolf, Eva
DOI：
10.1073/pnas.0409575102
发表时间：
2005-06-07
影响因子：
11.1
作者：
Mouritsen, H;Feenders, G;Jarvis, ED
通讯作者：
Jarvis, ED
影响因子：
3.4
作者：
Ritz, T;Adem, S;Schulten, K
通讯作者：
Schulten, K