Pattern-specific associative long-term potentiation induced by a sleep spindle-related spike train
Pattern-specific associative long-term potentiation induced by a sleep spindle-related spike train
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DOI：
10.1523/jneurosci.2149-05.2005
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发表时间：
2005-10-12
影响因子：
5.3
通讯作者：
Ulrich, D
中科院分区：
文献类型：
--
作者：
Rosanova, M;Ulrich, D
Spectrometry是非快速眼动（非REM）睡眠EEG节律（7 - 14 Hz），其独立发生或与缓慢振荡（0.6 - 0.8 Hz）相关。尽管它们在学习和记忆中的功能被提出，但它们在突触可塑性中的作用基本上是未知的。我们研究了在体外新皮层锥体细胞中，体内纺锤体神经元放电模式诱导突触可塑性的能力。从记录在用氯胺酮-甲苯噻嗪麻醉的猫中的缓慢振荡upstate中提取纺锤体刺激模式（SSP），已知氯胺酮-甲苯噻嗪诱导睡眠样状态。为了模拟由缓慢振荡分组的纺锤体的重现，每1.5秒（0.6 Hz）重复SSP。用红外视频显微镜对大鼠体感皮层第V层锥体细胞进行全细胞膜片钳记录，并在第II ~ III层诱发复合EPSP。由SSP同时触发的一系列EPSP和动作电位可诱导NMDA受体依赖的短时程增强（STP）和L型钙通道依赖的长时程增强（LTP）。纺锤体序列的数目影响STP-LTP的表达量。相比之下，EPSP的纺锤体序列单独导致长期抑郁。LTP并不总是由规则的放电模式、镜像SSP或随机SSP诱导;然而，由10 Hz重复性尖峰脉冲组成的合成纺锤体模式可靠地诱导STP-LTP。我们的研究结果表明，纺锤体相关的锋电位放电是有效的修改兴奋性新皮层突触根据赫布规则。这支持了睡眠纺锤波在记忆巩固中的作用。
Spindles are non-rapid eye movement (non-REM) sleep EEG rhythms (7 - 14 Hz) that occur independently or in association with slow oscillations (0.6 - 0.8 Hz). Despite their proposed function in learning and memory, their role in synaptic plasticity is essentially unknown. We studied the ability of a neuronal firing pattern underlying spindles in vivo to induce synaptic plasticity in neocortical pyramidal cells in vitro. A spindle stimulation pattern (SSP) was extracted from a slow oscillation upstate that was recorded in a cat anesthetized with ketamine-xylazine, which is known to induce a sleep-like state. To mimic the recurrence of spindles grouped by the slow oscillation, the SSP was repeated every 1.5 s (0.6 Hz). Whole-cell patch- clamp recordings were obtained from layer V pyramidal cells of rat somatosensory cortex with infrared videomicroscopy, and composite EPSPs were evoked within layers II - III. Trains of EPSPs and action potentials simultaneously triggered by the SSP induced an NMDA receptor-dependent short-term potentiation (STP) and an L-type Ca2+ channel-dependent long-term potentiation (LTP). The number of spindle sequences affected the amount of STP-LTP. In contrast, spindle trains of EPSPs alone led to long-term depression. LTP was not consistently induced by a regular firing pattern, a mirrored SSP, or a randomized SSP; however, a synthetic spindle pattern consisting of repetitive spike bursts at 10 Hz reliably induced STP-LTP. Our results show that spindle-associated spike discharges are efficient in modifying excitatory neocortical synapses according to a Hebbian rule. This is in support of a role for sleep spindles in memory consolidation.