The analysis of carbohydrates in milk powder by a new "heart-cutting" two-dimensional liquid chromatography method
The analysis of carbohydrates in milk powder by a new "heart-cutting" two-dimensional liquid chromatography method
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DOI：
10.1016/j.jpba.2013.11.006
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发表时间：
2014-03-25
影响因子：
3.4
通讯作者：
He, Langchong
中科院分区：
文献类型：
--
作者：
Ma, Jing;Hou, Xiaofang;He, Langchong
建立了一种同时测定奶粉中碳水化合物含量的“切心”二维液相色谱法。在二维液相色谱系统中，第一维（D-1）采用Venusil XBP-C4分析柱作为预分离柱，第二维（D-2）采用ZORBAX碳水化合物分析柱作为终分析柱。整个过程在不到35分钟内完成，没有特殊的样品制备程序。用二维高效液相色谱法测定了不同品牌奶粉样品中碳水化合物的含量。提出了一种常规的一维色谱法。两种方法在线性度、检出限、准确度和精密度方面均得到了验证。两种方法的结果比较表明，新型的全自动二维液相色谱法具有在线净化效果，更适合于奶粉样品。爱思唯尔B.V.版权所有
In this study, a new"heart-cutting" two-dimensional liquid chromatography method for the simultaneous determination of carbohydrate contents in milk powder was presented. In this two dimensional liquid chromatography system, a Venusil XBP-C4 analysis column was used in the first dimension (D-1) as a pre-separation column, a ZORBAX carbohydrates analysis column was used in the second dimension (D-2) as a final-analysis column. The whole process was completed in less than 35 min without a particular sample preparation procedure. The capability of the new two dimensional HPLC method was demonstrated in the determination of carbohydrates in various brands of milk powder samples. A conventional one dimensional chromatography method was also proposed. The two proposed methods were both validated in terms of linearity, limits of detection, accuracy and precision. The comparison between the results obtained with the two methods showed that the new and completely automated two dimensional liquid chromatography method is more suitable for milk powder sample because of its online cleanup effect involved. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.