Prokaryotic expression and activity assay of recombinant chicken interferon-alpha.
Prokaryotic expression and activity assay of recombinant chicken interferon-alpha.
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发表时间：
2009
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通讯作者：
D. Hua;Zheng Jiayu;Chen Junhua;Pan Zhiming;J. Xin-an
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作者：
D. Hua;Zheng Jiayu;Chen Junhua;Pan Zhiming;J. Xin-an
Chicken IFN-αgene was amplified by RT-PCR and cloned from chicken spleen lymphocytes stimulated by concanavalin A.Recombinant plasmids (pGEX-proChIFN-α,pET-ChIFN-α) were expressed in E.coli.SDS-PAGE analysis showed that the expression products had a molecular weight of 45 ku and 25 ku respectively,and existed in inclusion bodies.The expressed protein was shown to inhibit H5N1 avian influenza virus or vesicular stomatitis virus replication on chick embryo fibroblast (CEF) at 2×106 u/mg.The potential of chicken IFN-α as adjuvant was discussed.
