Shiga-toxin producing Escherichia coli from healthy cattle in a semi-urban community in Calcutta, India.
Shiga-toxin producing Escherichia coli from healthy cattle in a semi-urban community in Calcutta, India.
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印度加尔各答城郊社区的健康牛身上产生志贺毒素大肠杆菌。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Y. Takeda
中科院分区：
文献类型：
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作者：
A. Pal;Santanu Ghosh;T. Ramamurthy;Shinji Yamasaki;T. Tsukamoto;S. Bhattacharya;Nair Gb;Y. Takeda
1998 年 10 月至 12 月，对加尔各答附近城郊社区 67 头健康牛的单一粪便样本进行了产志贺毒素埃希菌的检查。使用多重 PCR 初步筛选大肠杆菌 (STEC)，然后在山梨糖醇 MacConkey 琼脂上进行平板接种。从 7 只 (10.5%) 动物的粪便中分离出 STEC。分离的 8 株菌株属于 8 个血清型，即 O146:H1、O149:HNT、ONT:H34、ONT:H19、O88:HN、ONT:H2、O82:H8 和 O28ac:H21。珠酶联免疫吸附试验显示，3株菌株产生志贺毒素1，1株菌株产生志贺毒素2，4株菌株同时产生两种毒素。
From October to December 1998, single faecal samples from 67 healthy cattle in a semi-urban community near Calcutta were examined for Shiga toxin producing Esch. coli (STEC) using a multiplex PCR primary screen followed by plating on sorbitol MacConkey agar. STEC was isolated from the faeces of 7 (10.5%) animals. The eight strains isolated belonged to eight serotypes viz, O146:H1, O149:HNT, ONT:H34, ONT:H19, O88:HN, ONT:H2, O82:H8 and O28ac:H21. Bead enzyme linked immunosorbent assay showed that three strains produced Shiga toxin 1, one produced Shiga toxin 2 and four produced both.