Plant adenosine 5′-phosphosulphate reductase:: the past, the present, and the future
Plant adenosine 5′-phosphosulphate reductase:: the past, the present, and the future
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DOI：
10.1093/jxb/erh185
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发表时间：
2004-08-01
影响因子：
6.9
通讯作者：
Koprivova, A
中科院分区：
文献类型：
--
作者：
Kopriva, S;Koprivova, A
硫酸盐同化途径为氨基酸半胱氨酸和蛋氨酸的合成提供了还原硫。它们是蛋白质的基本组成部分，也是合成辅酶和各种二级化合物所需的还原硫的进一步来源。最近的一些报告发现腺苷5'-磷酸硫酸还原酶（APR）是对该途径控制最大的酶。在这篇综述中，对硫酸盐同化的研究进行了简短的历史回顾，重点介绍了APR的替代途径，通过“结合亚硫酸盐”或通过PAPS还原酶。通过对APR和PAPS还原酶序列的系统发育分析，综述了APR的进化历史。此外，综述了近年来对APR的生化分析，包括铁硫中心作为辅助因子，提出了不同蛋白结构域的功能，并对酶的机制进行了探讨。最后，为了提高对APR分子机制和调控的理解，确定了必须解决的问题。
The sulphate assimilation pathway provides reduced sulphur for the synthesis of the amino acids cysteine and methionine. These are the essential building blocks of proteins and further sources of reduced sulphur for the synthesis of coenzymes and various secondary compounds. Several recent reports identified the adenosine 5'-phosphosulphate reductase (APR) as the enzyme with the greatest control over the pathway. In this review, a short historical excursion into the investigations of sulphate assimilation is given with emphasis on the proposed alternative pathways to APR, via 'bound sulphite' or via PAPS reductase. The evolutionary past of APR is reviewed, based on phylogenetic analysis of APR and PAPS reductase sequences. Furthermore, recent biochemical analyses of APR that identified an iron-sulphur centre as a cofactor, proposed functions for different protein domains, and addressed the enzyme mechanism are summarized. Finally, questions that have to be addressed in order to improve understanding of the molecular mechanism and regulation of APR have been identified.