Intra-specific heterogeneity of the rDNA internal transcribed spacer in the Simulium damnosum (Diptera: Simuliidae) complex.
Intra-specific heterogeneity of the rDNA internal transcribed spacer in the Simulium damnosum (Diptera: Simuliidae) complex.
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Simuliumdamnosum（双翅目：Simuliidae）复合体中 rDNA 内部转录间隔区的特异性内异质性。
DOI：
10.1093/oxfordjournals.molbev.a025561
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发表时间：
1996
影响因子：
10.7
通讯作者：
Unnasch,TR
中科院分区：
文献类型：
--
作者：
Tang,J;Toè,L;Back,C;Unnasch,TR
RRNA基因簇的内部转录间隔区(ITS)已被用作研究协同进化和分子驱动对重复序列家族作用的模型。与这一普遍发现相反，从西非黑蝇物种复合体Simulium Damnosum s.1克隆的ITS代表基因进行了初步的DNA序列分析。表现出广泛的个体内和种内多态。ITS的可变性主要限于ITS1领域。ITS内个体内和种内变异的程度和类型通过凝胶电泳、DNA杂交和从ITS1结构域产生的PCR产物的异源双链分析进一步表征。ITS1拷贝自丹参个体s.1。在长度和序列组成上不同。这些结果综合起来，说明丹参ITS中存在很大程度的个体内和种内异质性。栖息于稀树大草原的物种个体内异质性大于栖息于森林的丹参兄弟种1。这种异质性可能部分归因于同域兄弟种之间的异交，加上丹参的干扰。由于密集的病媒控制努力而产生的种群。
The internal transcribed spacer (ITS) of the rRNA gene cluster has been used as a model for the study of the action of concerted evolution and molecular drive on repeated sequence families. In contrast to this general finding, preliminary DNA sequence analysis of cloned representatives of the ITS from the West African black fly species complex Simulium damnosum s.1. demonstrated extensive intra-individual and intra-specific polymorphisms. Variability in the ITS was primarily confined to the ITS1 domain. The degree and type of intra-individual and intra-specific variability within the ITS was further characterized using gel electrophoresis, DNA hybridization, and heteroduplex analysis of the PCR products generated from the ITS1 domain. ITS1 copies from individual S. damnosum s.1. differed in length and sequence composition. These results, when taken together, demonstrate that a large degree of intra-individual and intra-specific heterogeneity exists in the ITS of S. damnosum s.1. The intra-individual heterogeneity was greater in the savanna-dwelling than forest-dwelling sibling species of S. damnosum s.1. This heterogeneity may be due in part to inter-breeding among sympatric sibling species, coupled with disturbance of S. damnosum s.1. populations resulting from intensive vector control efforts.