Laboratory-based surveillance of pertussis using multitarget real-time PCR in Japan: evidence for Bordetella pertussis infection in preteens and teens.
Laboratory-based surveillance of pertussis using multitarget real-time PCR in Japan: evidence for Bordetella pertussis infection in preteens and teens.
复制标题
DOI：
10.1016/j.nmni.2015.10.001
复制
发表时间：
2015-11
影响因子：
4
通讯作者：
Shibayama K
中科院分区：
文献类型：
--
作者：
Kamachi K;Yoshino S;Katsukawa C;Otsuka N;Hiramatsu Y;Shibayama K
2013年1月至2014年12月，采用多靶点实时荧光定量PCR技术对百日咳、副百日咳、霍尔默氏杆菌和肺炎支原体进行了实验室监测。在日本355例临床诊断为百日咳的患者中，百日咳b型、副百日咳b型和肺炎支原体检出率分别为26% （n = 94）、1.1% （n = 4）和0.6% (n = 2)，而霍氏b型未检出。经证实，百日咳双歧杆菌和肺炎支原体也可引起百日咳样疾病。百日咳的阳性率根据年龄从16%到49%不等。≤3个月的婴幼儿发病率最高（49%），1 ~ 4岁的婴幼儿发病率最低（16%，与≤3个月的婴幼儿相比p < 0.01）。10至14岁和15至19岁的人也显示出较高的阳性率（各29%）；与≤3月龄婴儿的阳性率比较，差异无统计学意义（p≥0.06）。我们的观察表明，与婴儿相似，青春期前和青少年是百日咳感染的高危人群。
Between January 2013 and December 2014, we conducted laboratory-based surveillance of pertussis using multitarget real-time PCR, which discriminates among Bordetella pertussis, Bordetella parapertussis, Bordetella holmesii and Mycoplasma pneumoniae. Of 355 patients clinically diagnosed with pertussis in Japan, B. pertussis, B. parapertussis and M. pneumoniae were detected in 26% (n = 94), 1.1% (n = 4) and 0.6% (n = 2), respectively, whereas B. holmesii was not detected. It was confirmed that B. parapertussis and M. pneumoniae are also responsible for causing pertussis-like illness. The positive rates for B. pertussis ranged from 16% to 49%, depending on age. Infants aged ≤ 3 months had the highest rate (49%), and children aged 1 to 4 years had the lowest rate (16%, p < 0.01 vs. infants aged ≤ 3 months). Persons aged 10 to 14 and 15 to 19 years also showed high positive rates (29% each); the positive rates were not statistically significant compared with that of infants aged ≤ 3 months (p ≥ 0.06). Our observations indicate that similar to infants, preteens and teens are at high risk of B. pertussis infection.