Emerging biomedical applications of time-resolved fluorescence spectroscopy
Emerging biomedical applications of time-resolved fluorescence spectroscopy
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DOI：
10.1117/12.180786
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发表时间：
1994-07
期刊：
影响因子：
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通讯作者：
J. Lakowicz;H. Szmacinski;P. A. Koen
中科院分区：
文献类型：
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作者：
J. Lakowicz;H. Szmacinski;P. A. Koen
时间分辨荧光光谱是目前生物化学、生物物理学和化学物理学的研究工具。激光技术的进步，长波长探针的开发以及基于寿命的方法的使用导致时间分辨荧光快速迁移到临床化学实验室，患者床边，流式细胞仪，医生办公室，甚至家庭医疗保健。此外，时间分辨成像现在是荧光显微镜的现实，并将提供各种细胞内分析物和/或细胞现象的化学成像。在这篇综述论文中，我们试图描述一些机会，使用基于荧光寿命的化学传感，并预测这些应用程序的寿命为基础的传感是最有可能在不久的将来。
Time-resolved fluorescence spectroscopy is presently regarded as a research tool in biochemistry, biophysics, and chemical physics. Advances in laser technology, the development of long-wavelength probes, and the use of lifetime-based methods are resulting in the rapid migration of time-resolved fluorescence to the clinical chemistry lab, to the patient's bedside, to flow cytometers, to the doctor's office, and even to home health care. Additionally, time-resolved imaging is now a reality in fluorescence microscopy, and will provide chemical imaging of a variety of intracellular analytes and/or cellular phenomena. In this overview paper we attempt to describe some of the opportunities available using chemical sensing based on fluorescence lifetimes, and to predict those applications of lifetime-based sensing which are most likely in the near future.