Screening of Oil-Producing Microalgae and Detecting Dynamics of Neutral Lipid Accumulation
Screening of Oil-Producing Microalgae and Detecting Dynamics of Neutral Lipid Accumulation
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发表时间：
2010
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通讯作者：
X. Jilin
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作者：
X. Jilin
微藻被认为是生产生物油的理想资源。采用尼罗红荧光法对宁波大学微藻保藏中心的63株微藻进行了中性脂质筛选，并对中性脂质积累动态进行了跟踪。产油量居前三位的藻种分别为海链藻、蛋白核小球藻和绿色巴夫藻。以尼罗红法为基础，观察了细胞中性脂质在种群生长过程中的变化。结果表明，中性脂肪从指数生长后期到稳定期开始大量积累。T.透射电子显微镜（TEM）也观察到，这进一步证实了中性脂质在生长后期的积累。表明T.因此，可以认为，微藻藻属是一种具有开发生物油潜力的微藻。
Microalgae are considered ideal resources for the production of bio-oil. In this study, 63 strains of microalgae, from Microalgae Collection Center of Ningbo University, were screened for neutral lipid by Nile red fluorescence, and dynamics of neutral lipid accumulation were tracked. The top three oil-producing algae were Thalassiosira pseudonana, Chlorella pyrenoidosa and Pavlova viridis. Based on the Nile red method, changes in cellular neutral lipid during population growth were observed. The results showed that neutral lipid began to accumulate abundantly from late exponential growth phase to stationary phase. Increase in lipid body in T. pseudonana was also observed by transmission election microscopy (TEM), which further confirmed accumulation of neutral lipid during late growth phase. It is indicated that T. pseudonana could be considered as potential microalgal species for bio-oil production.