Relationships between mitochondrial DNA content, mitochondrial activity, and boar sperm motility
Relationships between mitochondrial DNA content, mitochondrial activity, and boar sperm motility
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线粒体DNA含量、线粒体活性与公猪精子活力之间的关系
DOI：
10.1016/j.theriogenology.2016.09.005
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发表时间：
2017-01-01
期刊：
影响因子：
2.8
通讯作者：
Zhao, Ruqian
中科院分区：
文献类型：
--
作者：
Guo, Huiduo;Gong, Yabin;Zhao, Ruqian
线粒体通过氧化磷酸化 (OXPHOS) 产生的能量对于哺乳动物精子的活力至关重要。哺乳动物线粒体 DNA (mtDNA) 编码的蛋白质是 OXPHOS 系统的亚基。矛盾的是，活动精子中的线粒体和线粒体DNA含量比活动能力较差的精子要少。在这里，成熟的公猪精子被用作模型来研究 mtDNA 含量、线粒体活性和精子活力之间的关系。通过在不连续 Percoll 密度梯度上离心分离活动精子和活动性较差的精子。测定线粒体DNA的含量和表达以及线粒体活性和生物合成，以揭示可能的机制。与活动较少的精子相比，活动精子的线粒体 (P < 0.01) 和 mtDNA (P < 0.05) 含量较低。线粒体超微结构表明运动精子中线粒体活性较高，线粒体跨膜电位较高（P < 0.01），以及线粒体呼吸链复合物 I 活性较高（P < 0.05）。此外，更多的线粒体活性氧（P < 0.01）表明活动精子中的线粒体生物合成更高。尽管活动精子中线粒体DNA含量较低，伴随着转录因子表达较高，但在OXPHOS中起关键作用的线粒体DNA编码蛋白（细胞色素c氧化酶亚基1）的水平在活动精子中较高。这些结果有助于解释为什么无线粒体DNA的精子具有更高的线粒体活性和更好的运动性。 (C) 2016 Elsevier Inc. 保留所有权利。
Energy produced by mitochondria via oxidative phosphorylation (OXPHOS) is essential for mammalian sperm motility. Mammalian mitochondrial DNA (mtDNA)-encoded proteins are subunits of the OXPHOS system. Paradoxically, there are less mitochondrial and mtDNA contents in motile sperm than less motile sperm. Here, mature boar sperm was used as a model to investigate the relationships between mtDNA content, mitochondrial activity, and sperm motility. Motile and less motile sperm were separated by centrifugation on a discontinuous percoll density gradient. The contents and expression of mtDNA as well as mitochondrial activity and biosynthesis were determined to reveal possible mechanisms. Motile sperm showed less mitochondrial (P < 0.01) and mtDNA (P < 0.05) contents as compared to less motile sperm. Higher mitochondrial activity in motile sperm indicated by mitochondrial ultrastructure, higher mitochondrial transmembrane potential (P < 0.01), as well as higher mitochondrial respiratory chain complex I activity (P < 0.05). Moreover, more mitochondrial reactive oxygen species (P < 0.01) suggested higher mitochondrial biosynthesis in motile sperm. Although less mtDNA contents in motile sperm, accompanied by the higher expression of transcription factors, the level of mtDNA-encoded protein (cytochrome c oxidase subunit 1) which play pivotal role in OXPHOS was higher in motile sperm. The results are helpful to interpret why mtDNA-less sperm have higher mitochondrial activity and better motility. (C) 2016 Elsevier Inc. All rights reserved.