Inhibitory effect of a bioactivity-guided fraction from Rheum undulatum on the acid production of Streptococcus mutans biofilms at sub-MIC levels
Inhibitory effect of a bioactivity-guided fraction from Rheum undulatum on the acid production of Streptococcus mutans biofilms at sub-MIC levels
复制标题
DOI：
10.1016/j.fitote.2010.11.005
复制
发表时间：
2011-04-01
期刊：
影响因子：
3.4
通讯作者：
Jeon, Jae-Gyu
中科院分区：
文献类型：
--
作者：
Kim, Jeong-Eun;Kim, Hye-Jin;Jeon, Jae-Gyu
在韩国，大黄根传统上用于治疗牙科疾病。本研究的目的是从R.波形藻对变形链球菌生物膜显示出抗酸产生活性，并鉴定了该级分中的主要组分。R.制备了波形根及其级分。为了选择表现出抗酸产生活性的级分，进行悬浮糖酵解pH下降测定。在测试的馏分中，二氯甲烷馏分以剂量依赖性方式表现出最强的活性。考察所选组分对S. 74 h变形链球菌生物膜;使用变形菌生物膜。所选馏分降低了S的初始产酸速率。在亚最小抑菌浓度（MIC）水平下的变形杆菌生物膜。HPLC定性分析表明，芦荟大黄素、大黄素、大黄酚、大黄素甲醚等蒽醌类化合物为主要成分。(c)2010 Elsevier B. V.保留所有权利。
Rheum undulatum root has been used traditionally in Korea for the treatment of dental diseases. The purpose of this study was to separate a fraction from R. undulatum showing anti-acid production activity against Streptococcus mutans biofilms and identify the main components in that fraction. Methanol extract of R. undulatum root and its fractions were prepared. To select a fraction exhibiting anti-acid production activity, suspension glycolytic pH-drop assay was performed. Among the fractions tested, dichloromethane fraction exhibited the strongest activity in a dose-dependent manner. To examine the effect of the selected fraction on the anti-acid production of S. mutans biofilms, 74 h old S. mutans biofilms were used. The selected fraction reduced the initial rate of acid production of S. mutans biofilms at sub-minimum inhibitory concentration (MIC) levels. HPLC qualitative analysis of the selected fraction indicated that the presence of anthraquinone derivatives, such as aloe-emodin, emodin, chrysophanol and physcion, as main components. (c) 2010 Elsevier B.V. All rights reserved.