Methods to Analyze Motility in Eury- and Crenarchaea.
Methods to Analyze Motility in Eury- and Crenarchaea.
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分析 Eury- 和 Crenarchaea 运动性的方法
DOI：
10.1007/978-1-0716-2445-6_25
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发表时间：
2022
影响因子：
--
通讯作者：
Quax TEF
中科院分区：
文献类型：
--
作者：
Patro M;van Wolferen M;Albers SV;Quax TEF
许多古细菌在液体介质中表现出游泳运动性，这是由古细菌赋予的。定向运动需要一个功能性的古菌和一个传感系统，如广古菌所使用的趋化系统。两个研究得很好的模型是euryarchaeonHaloferax volcanii和crenarchaeonSulfolobus acidocaldarius。在本章中，我们描述了两种方法来分析他们的游泳行为和定向运动：（a）在自然温度下的延时显微镜和（B）在半固体琼脂或凝胶平板上斑点。第一种方法允许对游泳行为进行深入分析，而第二种方法适用于多种菌株的高通量比较。
Many archaea display swimming motility in liquid medium, which is empowered by the archaellum. Directional movement requires a functional archaellum and a sensing system, such as the chemotaxis system that is used by Euryarchaea. Two well-studied models are the euryarchaeonHaloferax volcaniiand the crenarchaeonSulfolobus acidocaldarius. In this chapter we describe two methods to analyze their swimming behavior and directional movement: (a) time-lapse microscopy under native temperatures and (b) spotting on semi-solid agar or gelrite plates. Whereas the first method allows for deep analysis of swimming behavior, the second method is suited for high throughput comparison of multiple strains.