Nucleotide Sequence of the Cyclomaltodextrin Glucanotransferase (CGTase) Gene from Alkalophilic Bacillus sp. Strain No. 38-2
Nucleotide Sequence of the Cyclomaltodextrin Glucanotransferase (CGTase) Gene from Alkalophilic Bacillus sp. Strain No. 38-2
复制标题
嗜碱芽孢杆菌属环麦芽糖糊精葡聚糖转移酶 (CGTase) 基因的核苷酸序列。
DOI：
10.1271/bbb1961.51.2019
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
K. Horikoshi
中科院分区：
文献类型：
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作者：
T. Hamamoto;T. Kaneko;K. Horikoshi
环糊精（CD）是一系列同源的非还原性环状D-葡萄糖聚合物，在一个环结构中含有六个或更多个糖基。1}这些糊精是由淀粉和相关材料通过环麦芽糖糊精葡聚糖转移酶（CGTase，EC 2.4.1.19）合成的。这些酶由多种微生物产生。2 ~6）嗜碱芽孢杆菌菌株No.38 -2产生一种细胞外CGTase，引领了环糊精工业。我们用pBR 322在大肠杆菌中克隆了嗜碱芽孢杆菌38-2号菌株的CGTase基因，用Sau 3AI部分切割该菌株的染色体DNA，将片段连接到BamHI酶切的pBR 322上。携带该质粒的质粒具有CGT酶活性。
The cyclodextrins (CD) are a homologous series ofnon- reducing, cyclic, D-glucose polymers with six or more glycosyls in a ring structure.1} These dextrins are synthe-sized from starch and related materials by cyclomaltodex- trin glucanotransferase (CGTase, EC 2.4.1.19). These enzymes are produced by several microorganisms.2~6) Alkalophilic Bacillus sp. strain No. 38-2 produces an extracellular CGTase which led the cyclodextrin industries. We have cloned the CGTase gene of alkalophilic Bacillus sp. strain No. 38-2 in Escherichia coli with pBR322to enzymatic The chromosomal DNAof strain was partially cleaved with Sau3AI and the fragments were ligated to BamHl digested pBR322. A transformant carrying the plasmid the has the CGTase activity.