Preparation and dynamic viscoelasticity characterization of alkali-solubilized collagen from shark skin.
Preparation and dynamic viscoelasticity characterization of alkali-solubilized collagen from shark skin.
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DOI：
10.1021/jf990389d
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发表时间：
2000-02
影响因子：
6.1
通讯作者：
K. Yoshimura;M. Terashima;D. Hozan;K. Shirai
中科院分区：
文献类型：
--
作者：
K. Yoshimura;M. Terashima;D. Hozan;K. Shirai
通过胶原溶液（10 mg/mL）的动态粘弹性测量来表征通过碱-酸提取和碱直接提取（分别缩写为AASC和ALSC）制备的碱溶性胶原。就产率和多肽大小而言，最佳制备条件如下：对于碱-酸提取，在20 ℃下用含有15%Na（2）SO（4）的0.5或1 M NaOH预处理5天，随后进行酸提取，对于碱直接提取，在4 ℃下用含有10%NaCl的0.5M NaOH处理20-30天。AASC和ALSC的多肽大小的主要部分由α链（α 1和α 2）组成。动态粘弹性的胶原蛋白溶液作为温度的函数进行测量。AASC表现出更大的贡献的弹性行为，而不是粘性行为。相反，ALSC表现出更强的粘性行为比弹性行为。
Alkali-solubilized collagens, prepared by alkali-acid extraction and alkali direct extraction (abbreviated AASC and ALSC, respectively), were characterized by dynamic viscoelastic measurement of collagen solution (10 mg/mL). The optimum preparative conditions in terms of yield and polypeptide size are as follows: for the alkali-acid extraction, a pretreatment with 0.5 or 1 M NaOH containing 15% Na(2)SO(4) within 5 days at 20 degrees C followed by the subsequent acid extraction, and for the alkaline direct extraction, a treatment with 0.5 M NaOH containing 10% NaCl at 4 degrees C for 20-30 days. A major portion of the polypeptide sizes of AASC and ALSC is composed of alpha chains (alpha1 and alpha2). Dynamic viscoelasticity of collagen solution was measured as a function of temperature. AASC showed a greater contribution of elastic behavior rather than viscous behavior. On the contrary, ALSC exhibits a stronger viscous behavior than elastic behavior.