Filamentous bacteriophage stability in non-aqueous media
Filamentous bacteriophage stability in non-aqueous media
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DOI：
10.1016/s1074-5521(01)00041-2
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发表时间：
2001-07-01
影响因子：
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通讯作者：
Nicholls, IA
中科院分区：
文献类型：
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作者：
Olofsson, L;Ankarloo, J;Nicholls, IA
背景：丝状噬菌体作为一般的克隆载体和噬菌体展示载体用于研究大肠杆菌的表达。与受体的相互作用暴露于双相氯仿-水界面导致噬菌体的特异性收缩，非感染性的I-或S-forms.Results：暴露后。噬菌体在甲醇、乙醇和异丙醇浓度下被灭活（非感染性），这与醇的疏水性成反比。在一定浓度的I-丙醇或乙醇的噬菌体的免疫力损失与在紫外荧光和圆二色study.Conclusions的光谱特性的变化相吻合的fl病毒粒子：醇类的丝状噬菌体的一般机制-溶剂化的外壳蛋白-从而破坏衣壳的方式完全不同的，从以前报道的I-和S-形式。噬菌粒pG 8H 6在99%乙腈中的感染性保留和此处研究的噬菌体菌株的相对高的一般溶剂抗性开辟了在非水介质中采用噬菌体展示的可能性。(C)2001爱思唯尔科技有限公司版权所有。
Background: Filamentous bacteriophage are used as general cloning vectors as well as phage display vectors in order to study li.-and-receptor interactions. Exposure to biphasic chloroform-water interface leads to specific contraction of phage, to noninfective I- or S-forms.Results: Upon exposure. phage were inactivated (non-infective) at methanol, ethanol and I-propanol concentrations inversely dependent upon alcohol hydrophobicity. Infectivity loss of phage at certain concentrations of I-propanol or ethanol coincided with changes in the spectral properties of the fl virion in ultraviolet fluorescence and circular dichroism studies.Conclusions: The alcohols inactivate filamentous phage by a general mechanism - solvation of coat protein - thereby disrupting the capsid in a manner quite different from the previously reported I- and S-forms. The infectivity retention of phagemid pG8H6 in 99% acetonitrile and the relatively high general solvent resistance of the phage strains studied here open up the possibility of employing phage display in non-aqueous media. (C) 2001 Elsevier Science Ltd. All rights reserved.