Identification of specific protein amino acid substitutions of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli ST131: a proteomics approach using mass spectrometry
Identification of specific protein amino acid substitutions of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli ST131: a proteomics approach using mass spectrometry
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DOI：
10.1038/s41598-019-45051-z
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发表时间：
2019-06-12
影响因子：
4.6
通讯作者：
Hatano, Naoya
中科院分区：
文献类型：
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作者：
Nakamura, Akihiro;Komatsu, Masaru;Hatano, Naoya
产ESBL大肠杆菌的全球流行与序列类型131（ST 131）有关。然而，ST 131传播的机制仍不清楚。本研究寻找ST 131特异性氨基酸替换蛋白，并利用蛋白质组学分析来阐明ST 131的特征。五种蛋白质具有ST 131特异性氨基酸取代：未表征的蛋白质YahO与E34 A（m/z 7655）; UPF 0337蛋白质YjbJ与V59 D、D 60 S和T63 K（m/z 8351）;未表征的蛋白质YnfD与S106 T（m/z 8448）;和酸胁迫分子伴侣HdeA与Q92 K和N94 S（m/z 9714）。可溶性细胞色素b562（m/z 11783）显示7个氨基酸取代，并且大流行Glade的Glade C和非C之间的序列不同。计算机模拟分析显示YahO蛋白与YjbJ蛋白相互作用，可能与生物膜形成有关。虽然可溶性细胞色素b562的功能是未知功能的电子传递，但预测其参与生物膜形成。HdeA是一种胃酸抵抗相关蛋白。YnfD的功能完全不清楚。总之，ST 131特异性蛋白质氨基酸取代主要由胃酸抗性蛋白和功能未知的蛋白质（可能参与生物膜形成）组成，这可能是在人体肠道中长期定植的机制。
The global pandemic of ESBL-producing Escherichia coli is associated with sequence type 131 (ST131). However, mechanisms of ST131 spread remain unclear. This study searched for proteins with amino acid substitutions specific for ST131 and used proteomics analysis to clarify ST131 characteristics. Five proteins had ST131-specific amino acid substitutions: uncharacterized protein YahO with E34A (m/z 7655); UPF0337 proteinYjbJ with V59D, D60S and T63K (m/z 8351); uncharacterized protein YnfD with S106T (m/z 8448); and acid stress chaperone HdeA with Q92K and N94S (m/z 9714). Soluble cytochrome b562 (m/z 11783) showed seven amino acid substitutions, and the sequence differed between Glade C of the pandemic Glade and non-C. In silico analysis showed YahO protein-protein interaction with YjbJ, possibly related to biofilm formation. Although the function of soluble cytochrome b562 is electron transport of unknown function, its involvement in biofilm formation was predicted. HdeA was a gastric acid resistance-related protein. The function of YnfD was completely unclear. In conclusion, ST131-specific protein amino acid substitutions consisted mainly of a gastric acid resistance protein and proteins of unknown function (possibly involved in biofilm formation), which might be mechanisms for long-term colonization in the human intestinal tract.