Differential Requirements for Centrioles in Mitotic Centrosome Growth and Maintenance
Differential Requirements for Centrioles in Mitotic Centrosome Growth and Maintenance
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DOI：
10.1016/j.devcel.2019.06.004
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发表时间：
2019-08-05
影响因子：
11.8
通讯作者：
Dammermann, Alexander
中科院分区：
文献类型：
--
作者：
Cabral, Gabriela;Laos, Triin;Dammermann, Alexander
中心体是微管成核和锚定的主要场所，在动物细胞中协调纺锤体组装和细胞分裂。在有丝分裂开始时，中心粒在称为中心体成熟的过程中积累微管组织的中心粒周围物质（PCM）。中心体的成熟在多大程度上依赖于有丝分裂调节因子的持续活性或中心粒的存在，至今仍不清楚。利用C.在线虫早期胚胎中，我们发现PCM扩增需要Polo样激酶PLK-1和CEP 192（C. elegans），而不是其上游调节因子Aurora A（AIR-1），而PCM聚合物的维持仅依赖于PLK-1。SPD-2和PLK-1高度集中在中心粒。出乎意料的是，激光显微手术显示，虽然中心粒是PCM招聘和中心体结构完整性所必需的，但它们是PCM维护所必需的。我们提出了一个模型，中心粒促进中心体成熟的招聘PLK-1，但随后的维护发生通过PLK-1直接作用于PCM。
Centrosomes, the predominant sites of microtubule nucleation and anchorage, coordinate spindle assembly and cell division in animal cells. At the onset of mitosis, centrioles accumulate microtubule-organizing pericentriolar material (PCM) in a process termed centrosome maturation. To what extent centrosome maturation depends on the continued activity of mitotic regulators or the presence of centrioles has hitherto been unclear. Using the C. elegans early embryo, we show that PCM expansion requires the Polo-like kinase PLK-1 and CEP192 (SPD-2 in C. elegans), but not its upstream regulator Aurora A (AIR-1), while maintenance of the PCM polymer depends exclusively on PLK-1. SPD-2 and PLK-1 are highly concentrated at centrioles. Unexpectedly, laser microsurgery reveals that while centrioles are required for PCM recruitment and centrosome structural integrity they are dispensable for PCM maintenance. We propose a model whereby centrioles promote centrosome maturation by recruiting PLK-1, but subsequent maintenance occurs via PLK-1 acting directly within the PCM.