Propionibacterium acnes DNA detected in bronchoalveolar lavage cells from patients with sarcoidosis.
Propionibacterium acnes DNA detected in bronchoalveolar lavage cells from patients with sarcoidosis.
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发表时间：
2003-10
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通讯作者：
J. Hiramatsu;M. Kataoka;Y. Nakata;K. Okazaki;S. Tada;M. Tanimoto;Y. Eishi
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作者：
J. Hiramatsu;M. Kataoka;Y. Nakata;K. Okazaki;S. Tada;M. Tanimoto;Y. Eishi
背景和目的结节病的病因尚不清楚。痤疮丙酸杆菌是迄今为止在结节病淋巴结中发现的唯一细菌。我们试图检测结节病患者支气管肺泡灌洗（BAL）回收的细胞中的痤疮丙酸杆菌DNA。方法采用巢式聚合酶链反应（PCR）技术，对30例经病理证实的结节病患者和30例其他肺部疾病对照者的BAL细胞进行痤疮丙酸杆菌16 SrRNA检测。还通过原位PCR检查了来自三名最近的结节病患者和两名对照患者的BAL细胞以定位痤疮丙酸杆菌DNA。比较PCR阳性与阴性结节病患者的临床表现。结果21例（70%）结节病患者和7例（23%）对照者的BAL细胞中检测到痤疮丙酸杆菌DNA。在结节病患者肺泡巨噬细胞的细胞质中，有0.2%~ 2.8%的细胞质中可检测到痤疮丙酸杆菌DNA的原位信号，而在对照组的细胞质中未检测到痤疮丙酸杆菌DNA。在大约一半的携带痤疮丙酸杆菌DNA的结节病患者中发现肺实质吸收镓-67，但在其他结节病患者中没有发现。这些DNA阳性的患者中，胸部X线片上有更多的肺实质阴影，并且比其他结节病患者处于更晚期。结论：在确诊的结节病患者中，BAL细胞中检测到痤疮丙酸杆菌DNA明显更常见。检测与肺部疾病活动的某些指标相关。
BACKGROUND AND AIM OF THE WORK The causes of sarcoidosis are unknown. Propionibacterium acnes is so far the only bacterium to be found in sarcoid lymph nodes. We attempted to detect P. acnes DNA in cells recovered by bronchoalveolar lavage (BAL) from patients with sarcoidosis. METHODS BAL cells from 30 patients with histologically proven sarcoidosis and 30 controls with other lung diseases were examined by a nested polymerase chain reaction (PCR) for 16S rRNA of P. acnes. BAL cells from three recent sarcoid patients and two control patients were also examined by in situ PCR to locate P. acnes DNA. Clinical findings in sarcoid patients with and without positive results by PCR were compared. RESULTS P. acnes DNA was detected in BAL cells from 21 (70%) sarcoid patients and 7 (23%) control patients. In situ signals of P. acnes DNA were detected in the cytoplasm of 0.2% to 2.8% of alveolar macrophages from sarcoid patients, but from no cells of the control patients. Gallium-67 uptake by lung parenchyma was found in about half of the sarcoid patients with P. acnes DNA, but in none of the other sarcoid patients. More of these patients with such DNA had lung parenchymal shadows in chest X-ray films and were in more advanced stages of the disease than the other sarcoid patients. CONCLUSIONS Detection of P. acnes DNA in BAL cells was significantly more common in the patients with confirmed sarcoidosis. Detection was associated with some indices of disease activity in the lung.