A wheat protein kinase gene TaSnRK2.9-5A associated with yield contributing traits
A wheat protein kinase gene TaSnRK2.9-5A associated with yield contributing traits
复制标题
与产量贡献性状相关的小麦蛋白激酶基因TaSnRK2.9-5A
DOI：
10.1007/s00122-018-3247-7
复制
发表时间：
2019-04-01
影响因子：
5.4
通讯作者：
Jing, Ruilian
中科院分区：
文献类型：
--
作者：
Rehman, Shoaib Ur;Wang, Jingyi;Jing, Ruilian
为小麦产量相关性状的分子标记辅助选择，我们开发了育种者友好、高通量、高性价比的KASP标记。植物特异性蛋白激酶SnRK2s是一个与代谢调节、营养利用和对外界刺激的反应相关的主要信号基因家族。本研究从小麦(Triticum aestivum L.)5A、5B和5D染色体上分离到3个TaSnRK2.9拷贝。对TaSnRK2.9-5A、TaSnRK2.9-5B的编码区和TaSnRK2.9-5D的启动子区域进行序列多态性分析。TaSnRK2.9-5A存在单核苷酸多态(SNPs)，TaSnRK2.9-5B和TaSnRK2.9-5D未发现SNPs。TaSnRK2.9-5A的核苷酸序列全长2180个碱基，包含8个内含子和9个外显子。共检测到3个SNPs，分别位于308、698和1700nt。为了进行高通量的基因分型，我们开发了两个Kasp标记。在中国、欧洲和巴基斯坦的小麦群体中检测到4种单倍型Hap-5A-1、Hap-5A-2、Hap-5A-3和Hap-5A-4。关联分析采用Tassel(v 5.0)中的混合线性模型。结果表明，TaSnRK2.9-5A的Hap-5A-1/2与千粒重显著相关，Hap-5A-4与每穗粒数显著相关。超量表达的转基因水稻也表现出更高的穗粒数，这与关联分析结果一致。地理分布和等位基因频率表明，在中国(Hap-5A-1/2)、巴基斯坦(Hap-5A-1)、东欧(Hap-5A-1)和西欧(Hap-5A-4)小麦育种中，优先选择单倍型。这些结果表明，所开发的KASP标记可用于小麦育种中的标记辅助选择和产量改良。
We developed breeder-friendly high-throughput and cost-effective KASP marker for marker-assisted selection for grain yield related traits in wheat. Plant-specific protein kinase, SnRK2s, is a major family of signaling genes associated with metabolic regulations, nutrient utilization and response to external stimuli. In the present study, three copies of TaSnRK2.9 were isolated from chromosomes 5A, 5B and 5D of wheat (Triticum aestivum L.). The coding regions of TaSnRK2.9-5A, TaSnRK2.9-5B and promoter region of TaSnRK2.9-5D were investigated for sequence polymorphism. Single nucleotide polymorphisms (SNPs) were identified for TaSnRK2.9-5A, while no polymorphism was identified in TaSnRK2.9-5B and TaSnRK2.9-5D. The nucleotide sequence of TaSnRK2.9-5A consisted of 2180 bp having eight introns and nine exons. Three SNPs were identified at 308 nt, 698 nt and 1700 nt. For high-throughput genotyping, two kompetitive allele-specific PCR (KASP) markers were developed. Four haplotypes Hap-5A-1, Hap-5A-2, Hap-5A-3 and Hap-5A-4 were detected in wheat populations collected from China, Europe and Pakistan. Association analysis was performed with mixed linear model in TASSEL (v 5.0). The results indicated that Hap-5A-1/2 of TaSnRK2.9-5A were significantly associated with high thousand kernel weight, while Hap-5A-4 with high grains per spike. Overexpressing transgenic rice also showed higher grains per spike which is in accordance with association analysis results. Geographic distribution and allelic frequency indicted that the favored haplotypes were positively selected in Chinese (Hap-5A-1/2), Pakistani (Hap-5A-1), east European (Hap-5A-1) and west European (Hap-5A-4) wheat breeding. The results suggest that the developed KASP markers can be utilized in yield improvement by marker-assisted selection in wheat breeding.