Characteristics of fecal and mucosa-associated microbiota in Chinese patients with inflammatory bowel disease.
Characteristics of fecal and mucosa-associated microbiota in Chinese patients with inflammatory bowel disease.
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DOI：
10.1097/md.0000000000000051
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发表时间：
2014-08
期刊：
影响因子：
1.6
通讯作者：
Xia B
中科院分区：
文献类型：
--
作者：
Chen L;Wang W;Zhou R;Ng SC;Li J;Huang M;Zhou F;Wang X;Shen B;A Kamm M;Wu K;Xia B
肠道微生物群在炎症性肠病（IBD）的发病机制中发挥着重要作用，地理和遗传背景影响肠道微生物群的组成。然而，目前缺乏关于中国IBD患者粪便相关微生物群（FAM）和粘膜相关微生物群（MAM）整体变化和特征的证据。我们招募了 26 名克罗恩病 (CD) 患者、46 名溃疡性结肠炎 (UC) 患者和 21 名健康个体；我们从同一受试者中收集了匹配的新鲜粪便和粘膜样本。通过454-焦磷酸测序研究微生物群落。在 IBD 患者中观察到社区范围内 FAM 和 MAM 的变化。罗斯氏菌属、粪球菌属和瘤胃球菌属等几种产生丁酸盐的细菌的比例显着减少，而病原体埃希氏菌-志贺氏菌和肠球菌在 IBD 患者中普遍存在。 CD 和 UC 之间的 FAM 和 MAM 相似。在健康个体和 UC 患者中，FAM 与 MAM 不同。总之，IBD 患者的 FAM 和 MAM 成分发生了改变。丁酸产生菌的减少和机会致病菌的增加可能与IBD的发病有关。
The intestinal microbiota plays an important role in the pathogenesis of inflammatory bowel disease (IBD), and geographical and genetic backgrounds impact the composition of the intestinal microbiota. However, there is a lack of evidence regarding the overall changes and characteristics of fecal-associated microbiota (FAM) and mucosa-associated microbiota (MAM) in Chinese patients with IBD. We recruited 26 patients with Crohn’s disease (CD), 46 patients with ulcerative colitis (UC), and 21 healthy individuals; we collected matched fresh fecal and mucosal samples from the same subjects. The microbial communities were studied by 454-pyrosequencing. Community-wide changes in FAM and MAM were observed in patients with IBD. The proportion of several butyrate-producing bacteria, such as of the genera Roseburia, Coprococcus, and Ruminococcus were significantly reduced, whereas the pathogens Escherichia-Shigella and Enterococcus were prevalent in patients with IBD. FAM and MAM were similar between CD and UC. FAM differed from MAM in healthy individuals and patients with UC. In conclusion, the compositions of FAM and MAM were altered in patients with IBD. The reduction of butyrate-producing bacteria and the increase in opportunistic pathogens might be associated with the pathogenesis of IBD.