Redistribution of intermediate filament subunits during skeletal myogenesis and maturation in vitro.
Redistribution of intermediate filament subunits during skeletal myogenesis and maturation in vitro.
复制标题
DOI：
10.1083/jcb.82.2.577
复制
发表时间：
1979-08
期刊：
影响因子：
--
通讯作者：
Holtzer H
中科院分区：
文献类型：
--
作者：
Bennett GS;Fellini SA;Toyama Y;Holtzer H
采用免疫荧光法检测了体外骨骼肌管成熟过程中中间丝（IF）亚基的分布，使用了针对两种不同类型鸡IF亚基的抗体：(a)成纤维细胞的58-kdalton亚基（抗58k）和(b)平滑肌的55-kdalton亚基（抗55k）。抗- 58k结合到纤维丝网络在复制假定成肌细胞和成纤维细胞，以及未成熟的肌管。未成熟肌管呈纵丝状分布，遍布细胞质。随着成熟，肌管的抗- 58k染色减少，最终消失。Anti-55K选择性染色肌管，随着成熟，荧光定位在分布上发生了巨大的变化——从未成熟肌管中密集的纵向细丝到成熟肌管中与肌原纤维I- Z区相关的交叉条纹分布。然而，横纹肌抗55k模式的出现并不与横纹肌原纤维的出现一致，而是在之后的一段时间内发生。
The distribution of intermediate filament (IF) subunits during maturation of skeletal myotubes in vitro was examined by immunofluorescence, using antibodies against two different types of chick IF subunits: (a) 58-kdalton subunits of fibroblasts (anti-58K), and (b) 55-kdalton subunits of smooth muscle (anti-55K). Anti-58K bound to a filament network in replicating presumptive myoblasts and fibroblasts, as well as in immature myotubes. The distribution in immature myotubes was in longitudinal filaments throughout the cytoplasm. With maturation, staining of myotubes by anti-58K diminished and eventually disappeared. Anti-55K selectively stained myotubes, and the fluorescence localization underwent a drastic change in distribution with maturation--from dense, longitudinal filaments in immature myotubes to a cross-striated distribution in mature myotubes that was associated with the I--Z region of myofibrils. However, the emergence of a cross-striated anti-55K pattern did not coincide temperally with the emergence of striated myofibrils, but occurred over a period of days thereafter.