Amyloid precursor protein is synthesized by retinal ganglion cells, rapidly transported to the optic nerve plasma membrane and nerve terminals, and metabolized.
Amyloid precursor protein is synthesized by retinal ganglion cells, rapidly transported to the optic nerve plasma membrane and nerve terminals, and metabolized.
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淀粉样前体蛋白由视网膜神经节细胞合成，迅速转运至视神经质膜和神经末梢并代谢。
DOI：
10.1111/j.1471-4159.1993.tb02147.x
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发表时间：
1993
影响因子：
4.7
通讯作者：
Fine,RE
中科院分区：
文献类型：
--
作者：
Morin,PJ;Abraham,CR;Amaratunga,A;Johnson,RJ;Huber,G;Sandell,JH;Fine,RE
我们研究了在体内兔视网膜神经节细胞中β-淀粉样前体蛋白（APP）的合成、轴突运输和加工。这些CNS神经元通过构成视神经的轴突将视网膜连接到大脑。APP在视网膜神经节细胞中合成，并在小的运输囊泡中快速运输到视神经中。然后转移到轴突质膜以及神经末梢，并以小于5 h的f1/2代谢。注射[35 S]-甲硫氨酸、[35 S]半胱氨酸后5 h，在视神经中观察到C末端淀粉样蛋白或非淀粉样蛋白片段的显著蓄积，24 h后消失。视神经中APP的主要分子量种类为10110 kDa，并且是不含Kunitz蛋白酶抑制剂结构域的APP同种型。含有该序列的高分子量物质主要见于视网膜。在与APP相同的视神经膜隔室中存在一种蛋白酶，其可以潜在地切割APP以产生淀粉样蛋白生成片段。
We have investigated the synthesis, axonal transport, and processing of the β‐amyloid precursor protein (APP) in in vivo rabbit retinal ganglion cells. These CNS neurons connect the retina to the brain via axons that comprise the optic nerve. APP is synthesized in retinal ganglion cells and is rapidly transported into the optic nerve in small transport vesicles. It is then transferred to the axonal plasma membrane, as well as to the nerve terminals and metabolized with a f1/2of less than 5 h. A significant accumulation of C‐terminal amyloidogenic or nonamyloidogenic fragments is seen in the optic nerve 5 h after [35S]‐ methionine, [35S]cysteine injection, which disappears by 24 h. The major molecular mass species of APP in the optic nerve is ∼110 kDa, and is an APP isoform that does not contain a Kunitz protease inhibitor domain. Higher molecular mass species containing this sequence are seen mostly in the retina. A protease(s) that can potentially cleave APP to generate an amyloidogenic fragment is present in the same optic nerve membrane compartment as APP.