Genome sequence and comparative analysis of DRQ-2, the type strain of Nonomuraea indica.
Genome sequence and comparative analysis of DRQ-2, the type strain of Nonomuraea indica.
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DOI：
10.1016/j.ygeno.2020.03.023
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发表时间：
2020-03
期刊：
影响因子：
4.4
通讯作者：
M. Rao;Zhou-Yan Dong;Jian‐Yu Jiao;Yu Zhou;Jiao Zhao;Min Xiao;Wenjun Li
中科院分区：
文献类型：
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作者：
M. Rao;Zhou-Yan Dong;Jian‐Yu Jiao;Yu Zhou;Jiao Zhao;Min Xiao;Wenjun Li
菌株DRQ-2 T（Nomuraea indica的模式菌株）由于其能够产生多种工业上重要的酶如淀粉酶、天冬酰胺酶、纤维素酶、明胶酶、天冬酰胺酶和蛋白酶，因此具有基因组测序的价值。本文报道了DRQ-2 T菌株的基因组序列测定和比较。基因组大小估计为8，288，417（bp），由59个重叠群组成。基因组G + C含量为72.4%。用2个rRNA和64个tRNA共预测了7730个基因。对菌株DRQ-2 T的基因组分析表明，存在广泛的次级代谢产物基因簇。全基因组分析管道（PGAP）表明，DRQ-2 T菌株具有大量的独特基因。大多数N。indicaDRQ-2 T基因编码的是假想蛋白，表明这些直向同源物簇的功能仍有待确定。
Strain DRQ-2T(type strain ofNonomuraea indica) is worthy for genome sequencing, due to its ability to produce a wide variety of industrially important enzymes such as amylase, asparaginase, cellulase, gelatinase, glutaminase, and protease. Genome sequencing and comparison of strain DRQ-2Tis described in the present work. The genome size was estimated to be 8,288,417 (bp) that consisted of 59 contigs. The G + C content of the genome was 72.4%. A total of 7730 genes were predicted with two rRNAs and 64 tRNAs. The genome analysis of the strain DRQ-2Tshowed the presence of a wide range of secondary metabolite gene clusters. Pan-Genomes Analysis Pipeline (PGAP) indicated that strain DRQ-2Thad large numbers of unique genes. The majority ofN. indicaDRQ-2Tgenes encode for hypothetical proteins, indicating the functions of these ortholog clusters were still remain to be determined.