6S RNA regulates E-coli RNA polymerase activity
6S RNA regulates E-coli RNA polymerase activity
复制标题
DOI：
10.1016/s0092-8674(00)80873-9
复制
发表时间：
2000-06-09
期刊：
影响因子：
64.5
通讯作者：
Storz, G
中科院分区：
文献类型：
--
作者：
Wassarman, KM;Storz, G
三十多年前，人们发现了大肠杆菌6S核糖核酸，但它的功能仍然难以捉摸。在这里，我们证明了6S RNA以一种高度特异和有效的方式与RNA聚合酶结合。紫外光交联实验表明，6S RNA直接与RNA聚合酶的Sigma(70)和β/β‘亚基接触。当细胞进入生长稳定期时，6S RNA积累，并介导RNA聚合酶中生长阶段特有的变化。只有在6S RNA存在的情况下，才能观察到Sigma(70)与提取物中的核心RNA聚合酶之间的稳定联系。我们发现6S RNA在稳定期抑制依赖于sigma(70)的启动子的表达。我们的结果表明，6S RNA与RNA聚合酶的相互作用调节Sigma(70)-全酶活性。
The E. coli 6S RNA was discovered more than three decades ago, yet its function has remained elusive. Here, we demonstrate that 6S RNA associates with RNA polymerase in a highly specific and efficient manner. UV crosslinking experiments revealed that 6S RNA directly contacts the sigma(70) and beta/beta' subunits of RNA polymerase. 6S RNA accumulates as cells reach the stationary phase of growth and mediates growth phase-specific changes in RNA polymerase. Stable association between sigma(70) and core RNA polymerase in extracts is only observed in the presence of 6S RNA. We show 6S RNA represses expression from a sigma(70)-dependent promoter during stationary phase. Our results suggest that the interaction of 6S RNA with RNA polymerase modulates sigma(70)-holoenzyme activity.