Translation elongation factor 1-α gene from Pichia pastoris:: molecular cloning, sequence, and use of its promoter
Translation elongation factor 1-α gene from Pichia pastoris:: molecular cloning, sequence, and use of its promoter
复制标题
DOI：
10.1007/s00253-006-0698-6
复制
发表时间：
2007-03-01
影响因子：
5
通讯作者：
Lee, Hongweon
中科院分区：
文献类型：
--
作者：
Ahn, Jungoh;Hong, Jiyeon;Lee, Hongweon
从酵母毕赤酵母中克隆了编码翻译延伸因子1-α的基因。该基因揭示了一个1,380 bp的开放阅读框架，具有编码459个氨基酸的多肽的潜力，计算的质量为50.1 kDa。通过使用细菌脂肪酶基因作为报告基因，研究了启动子（P（TEF 1））在巴斯德毕赤酵母中的潜力，并与甘油醛-3-磷酸脱氢酶启动子（P（GAP））进行比较。P（TEF 1）表现出更紧密的生长相关的表达模式，在高葡萄糖浓度的存在下改善功能，以及产生重组蛋白的启动子活性，其水平类似于或在一种情况下大于P（GAP）。该基因的序列以登录号EF 014948保藏在GenBank中。
The gene encoding translation elongation factor 1-alpha from the yeast Pichia pastoris was cloned. The gene revealed an open reading frame of 1,380 bp with the potential to encode a polypeptide of 459 amino acids with a calculated mass of 50.1 kDa. The potential of the promoter (P (TEF1) ) in P. pastoris was investigated with comparison to the glyceraldehyde-3-phosphate dehydrogenase promoter (P (GAP) ) by using a bacterial lipase gene as a reporter gene. P (TEF1) demonstrated a tighter growth-associated expression mode, improved functioning in the presence of high glucose concentrations, and promoter activities that yielded recombinant protein at levels similar to or in one case greater than P (GAP) . The sequence of the gene was deposited in GenBank under accession no. EF014948.