Establishment and characterization of a new canine mast cell tumor cell line
Establishment and characterization of a new canine mast cell tumor cell line
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DOI：
10.1292/jvms.63.1031
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发表时间：
2001-09-01
影响因子：
1.2
通讯作者：
Fujinaga, T
中科院分区：
文献类型：
--
作者：
Ishiguro, T;Kadosawa, T;Fujinaga, T
从一只患有口腔粘膜肥大细胞瘤的3岁雄性杂种犬建立了一个新的细胞系（CoMS）。昏迷细胞在悬浮液中生长，倍增时间为27.0 +/- 0.7小时，细胞质颗粒对福尔马林敏感，在其超微结构发现中表现出不同的外观，并含有肝素蛋白聚糖和中性蛋白酶糜酶。钙离子载体A23187、P物质和伴刀豆球蛋白A引起CoMS细胞显著的组胺释放，而化合物48/80不能释放组胺。该细胞系将为犬肥大细胞瘤的研究提供一个可用的来源。
A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa. coma. cells grow in suspension with a doubling time of 27.0 +/- 0.7 hr, The cytoplasmic granules were formalin-sensitive, showed diverse appearances in their ultrastructural findings and contained heparin proteoglycan and neutral protease chymase. Calcium ionophore A23187, substance P and concanavalin A caused significant histamine release from CoMS cells, while compound 48/80 failed to release histamine. This cell line will make an available source for studies on canine mast cell tumors.