IMMUNOGOLD LOCALIZATION OF THE DNAK HEAT-SHOCK PROTEIN IN ESCHERICHIA-COLI-CELLS
IMMUNOGOLD LOCALIZATION OF THE DNAK HEAT-SHOCK PROTEIN IN ESCHERICHIA-COLI-CELLS
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DOI：
10.1099/00221287-139-1-95
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
WALKER, GC
中科院分区：
文献类型：
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作者：
BUKAU, B;REILLY, P;WALKER, GC
先前报道的细胞分离实验对大肠杆菌DnaK热休克蛋白的细胞定位产生了相互矛盾的信息。在这里，我们使用免疫金标记超薄切片来确定30℃下非应激细胞和热休克细胞中DnaK的定位。在30℃条件下生长的细胞中，金颗粒主要存在于细胞质中，表明大多数DnaK分子存在于细胞质中；然而，一小部分金颗粒位于膜附近，这增加了DnaK蛋白亚群与膜相关的可能性。细胞热休克未引起可检测到的DnaK的重新定位。
Previously reported cell fractionation experiments have yielded conflicting information on the cellular localization of the DnaK heat shock protein of Escherichia coli. Here we used immunogold labelling of ultra-thin sections to determine the localization of DnaK in unstressed cells at 30-degrees-C as well as in heat-shocked cells. In cells grown at 30-degrees-C, gold particles were found predominantly in the cytoplasm, indicating that the majority of the DnaK molecules are cytoplasmic; however, a fraction of the gold particles was located in proximity to the membranes, raising the possibility that a subpopulation of DnaK proteins is membrane-associated. Heat shock of the cells did not induce detectable relocalization of DnaK.