The cohesion protein MEI-S332 localizes to condensed meiotic and mitotic centromeres until sister chromatids separate.
The cohesion protein MEI-S332 localizes to condensed meiotic and mitotic centromeres until sister chromatids separate.
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DOI：
10.1083/jcb.140.5.1003
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发表时间：
1998-03-09
期刊：
影响因子：
--
通讯作者：
Orr-Weaver TL
中科院分区：
文献类型：
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作者：
Moore DP;Page AW;Tang TT;Kerrebrock AW;Orr-Weaver TL
果蝇MEI-S332蛋白已被证明是维持雄性和雌性减数分裂中姐妹染色单体内聚所必需的。在雄性减数分裂过程中，当姐妹染色单体附着在着丝粒上时，这种蛋白质就定位在着丝粒上，在它们分离后就无法检测到。果蝇雄性减数分裂在几个方面是不典型的，因此确定雌性减数分裂期间MEI-S332的行为很重要，这更好地代表了真核生物的减数分裂。我们发现MEI-S332定位于卵母细胞前期I染色体的着丝粒，直到后期II去定位。通过使用卵母细胞，我们能够获得足够的材料来研究MEI-S332在II中期到II后期转变后的命运。MEI-S332蛋白的水平在减数分裂完成后保持不变，即使翻译被阻断，这表明该蛋白与着丝粒分离，但在后期II开始时不会被降解。出乎意料的是，MEI-S332在胚胎发生过程中存在，定位于有丝分裂染色体的着丝粒上，并从后期染色体上脱位。因此，MEI-S332与减数分裂和有丝分裂染色体的着丝粒结合，并在后期与它们分离。
The Drosophila MEI-S332 protein has been shown to be required for the maintenance of sister-chromatid cohesion in male and female meiosis. The protein localizes to the centromeres during male meiosis when the sister chromatids are attached, and it is no longer detectable after they separate. Drosophila melanogaster male meiosis is atypical in several respects, making it important to define MEI-S332 behavior during female meiosis, which better typifies meiosis in eukaryotes. We find that MEI-S332 localizes to the centromeres of prometaphase I chromosomes in oocytes, remaining there until it is delocalized at anaphase II. By using oocytes we were able to obtain sufficient material to investigate the fate of MEI-S332 after the metaphase II–anaphase II transition. The levels of MEI-S332 protein are unchanged after the completion of meiosis, even when translation is blocked, suggesting that the protein dissociates from the centromeres but is not degraded at the onset of anaphase II. Unexpectedly, MEI-S332 is present during embryogenesis, localizes onto the centromeres of mitotic chromosomes, and is delocalized from anaphase chromosomes. Thus, MEI-S332 associates with the centromeres of both meiotic and mitotic chromosomes and dissociates from them at anaphase.