Necrosis and apoptosis in neuroretina and pigment epithelium after diffuse photodynamic action in rats: a light and electron microscopic study.
Necrosis and apoptosis in neuroretina and pigment epithelium after diffuse photodynamic action in rats: a light and electron microscopic study.
复制标题
大鼠弥散光动力作用后神经视网膜和色素上皮的坏死和凋亡：光和电子显微镜研究。
DOI：
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发表时间：
1996
影响因子：
2.4
通讯作者：
P. Szczęsny
中科院分区：
文献类型：
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作者：
E. R. Büchi;P. Szczęsny
研究表明，压力引起的缺血后，大鼠视网膜中的细胞死亡主要沿着两条不同的途径发生。作者进行了一项定性研究，以确定在弥漫性光动力损伤和光血栓形成后是否可以识别出类似类型的细胞死亡。静脉注射后注射孟加拉玫瑰红 (40 mg/kg)，将 21 只白化大鼠的眼睛暴露在光线下，持续时间为 2 至 15 分钟，光强度为 15-17,000 ft-cd，并插入 Kodak Wratten 明胶滤光片 #15。存活时间从 15 分钟到 6 天不等。通过光学和电子显微镜研究样本。我们在神经视网膜和视网膜色素上皮（RPE）中发现了两种类型的视网膜细胞死亡。 I型细胞死亡与坏死一致，而II型细胞死亡则表现出细胞凋亡的许多特征。这些观察可能并不详尽。该模型中视网膜细胞死亡的模式似乎与压力引起的缺血后发现的相同。 RPE 细胞凋亡的形态学迄今尚未描述。这项研究证实了暴露于各种有害刺激后视网膜中有限数量的细胞死亡的概念。
Cell death in the rat retina has been shown to occur mainly along two distinct pathways after pressure-induced ischemia. The authors conducted a qualitative investigation to determine whether similar types of cell death could be identified after diffuse photodynamic injury and photothrombosis. After i.v. injection of rose bengal (40 mg/kg), the eyes of 21 albino rats were exposed to light for durations ranging from 2 to 15 minutes, at a light intensity of 15-17,000 ft-cd, with interposition of a Kodak Wratten gelatin filter #15. Survival times ranged from 15 minutes to 6 days. The specimens were studied by light and electron microscopy. We identified two types of retinal cell death in the neuroretina and in the retinal pigment epithelium (RPE). Type I cell death was consistent with necrosis, and type-II cell death showed many features of apoptosis. These observations may not be exhaustive. The patterns of retinal cell death in this model appeared to be identical with those found after pressure-induced ischemia. The morphology of apoptosis of the RPE is hitherto undescribed. This study corroborates the concept of a limited number of types of cell death in the retina after exposure to noxious stimuli of various kinds.