PACAP in visceral afferent nerves supplying the rat digestive and urinary tracts
PACAP in visceral afferent nerves supplying the rat digestive and urinary tracts
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DOI：
10.1111/j.1749-6632.1998.tb11233.x
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Hannibal, J
中科院分区：
文献类型：
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作者：
Fahrenkrug, J;Hannibal, J
PACAP是血管活性肠肽（VIP）家族的新成员，存在于脑2 -7以及许多外周器官的神经元元件中。8-12 PACAP在背根神经节、三叉神经节13-14和中脑三叉神经核16的神经细胞体中表达，表明该肽具有感觉作用。在本研究中，我们确定了PACAP感觉神经纤维对大鼠内脏器官神经支配的贡献，方法是：（1）用放射免疫测定法和免疫组织化学法检测新生儿用感觉神经毒素辣椒素处理对PACAP免疫反应性的影响，（2）用PACAP和降钙素基因相关肽（CGRP）的双重免疫染色，一种已知储存在辣椒素敏感的感觉神经中的肽。
PACAP is a new member of the vasoactive intestinal polypeptide (VIP) family of pep-tides1 present in the brain2–7 as well as in neuronal elements of a number of peripheral organs. 8–12 PACAP is expressed in nerve cell bodies of the dorsal root ganglia, 13–14 trigeminal ganglion, 15 and mesencephalic trigeminal nucleus, 16 suggesting a sensory role for the peptide. In the present study we have determined the contribution of PACAP sensory nerve fibers to the innervation of rat visceral organs by (1) examining the effect of neonatal treatment with the sensory neurotoxin capsaicin on PACAP immunoreactivity, determined by radioimmunoassay and immunohistochemistry, and (2) using double-immunostaining for PACAP and calcitonin gene–related peptide (CGRP), a peptide known to be stored in capsaicin-sensitive sensory nerves.