DETECTION OF POLYPEPTIDES ASSOCIATED WITH THE HISTOPATHOLOGICAL DIFFERENTIATION OF PRIMARY LUNG-CARCINOMA
DETECTION OF POLYPEPTIDES ASSOCIATED WITH THE HISTOPATHOLOGICAL DIFFERENTIATION OF PRIMARY LUNG-CARCINOMA
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DOI：
10.1038/bjc.1995.422
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发表时间：
1995-10-01
影响因子：
8.8
通讯作者：
AUER, G
中科院分区：
文献类型：
--
作者：
HIRANO, T;FRANZEN, B;AUER, G
二维聚丙烯酰胺凝胶电泳结合非酶样制备技术对临床肿瘤材料分析是有用的。使用这些技术，我们分析了原发性肺恶性肿瘤的组织病理学结果与在分子量20-35 kDa区域检测到的一些未识别多肽的表达之间的关系。本文报告45例原发性肺癌（腺癌21例，鳞状细胞癌10例，大细胞癌5例，腺鳞状细胞癌1例，小细胞癌5例，类癌3例）。作为参考，采用人二倍体成纤维细胞系WI38和正常外周血淋巴细胞。16个多肽被认为与组织病理学特征有关。这些多肽似乎是有价值的分化标记。同时评估这些多肽和一些其他增殖标志物（如PCNA、PCNA ‘卫星’、Numatin/protein B23和lamin B）似乎可以阐明每个PLC病例的特征。此外，可以根据二维电泳结果对PLC进行分类，并且建议PLC的这种分类比仅基于形态学的分类更准确地反映肿瘤的生物学特征。
Two-dimensional polyacrylamide gel electrophoresis combined with a non-enzymatic sample preparation technique is useful for analysing clinical tumour material. Using these techniques, we analysed the relationship between the histopathological findings in primary lung malignancies and the expression of a number of unidentified polypeptides that were detected in the molecular weight region 20-35 kDa. In this study 45 cases of primary lung cancer (PLC) (21 cases of adenocarcinoma, ten cases of squamous cell carcinoma, five cases of large-cell carcinoma, one case of adenosquamous cell carcinoma, five cases of small-cell carcinoma and three cases of carcinoid tumour) were examined. For reference, a human diploid fibroblast cell line (WI38) and normal peripheral lymphocytes were used. Sixteen polypeptides were judged to be associated with histopathological features. These polypeptides seem to be valuable as differentiation markers. The simultaneous evaluation of these polypeptides and some other proliferation markers (e.g. PCNA, PCNA 'satellite', Numatin/protein B23 and lamin B) seems to clarify the characteristics of each case of PLC. Furthermore, it is possible to classify PLC based on the two-dimensional electrophoresis findings, and this classification of PLC is suggested to reflect the biological features of the tumour more precisely than that based only on morphology.