In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.
In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.
复制标题
使用直流场刺激的周围神经的体外电泳和体内电生理学。
DOI：
10.1016/j.jneumeth.2014.01.018
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发表时间：
2014
影响因子：
3
通讯作者：
Wilson,WilkieA
中科院分区：
文献类型：
--
作者：
Madison,RogerD;Robinson,GrantA;Krarup,Christian;Moldovan,Mihai;Li,Qiang;Wilson,WilkieA
Backgroundgiven运动的分子在组织内发生的内源性电场，我们研究了使用低压直流电场的可能性，以移动带电物质在啮齿类动物外周神经vitrial.New方法标记的糖和蛋白质为基础的标记物施加到啮齿类动物腓神经，然后5-10 V/cm的字段被用来移动的分子内的外和神经内的隔间。生理和解剖的神经特性也进行了评估，使用相同的stimulationin vivo.ResultsWe demonstratinvitrothat带电和标记的化合物能够移动在直流电场沿着神经，和相同的领域appliedin vivo的神经的兴奋性的变化，但没有damage.ConclusionsThe结果表明，低压电泳可用于移动带电分子，也许治疗，安全沿着周围神经。
BackgroundGiven the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nervein vitro.New methodLabeled sugar- and protein-based markers were applied to a rodent peroneal nerve and then a 5–10 V/cm field was used to move the molecules within the extra- and intraneural compartments. Physiological and anatomical nerve properties were also assessed using the same stimulationin vivo.ResultsWe demonstratein vitrothat charged and labeled compounds are capable of moving in a DC field along a nerve, and that the same field appliedin vivochanges the excitability of the nerve, but without damage.ConclusionsThe results suggest that low-voltage electrophoresis could be used to move charged molecules, perhaps therapeutically, safely along peripheral nerves.