TOP mRNAs are translationally inhibited by a titratable repressor in both wheat germ extract and reticulocyte lysate
TOP mRNAs are translationally inhibited by a titratable repressor in both wheat germ extract and reticulocyte lysate
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DOI：
10.1016/s0014-5793(99)00983-7
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发表时间：
1999-08-13
期刊：
影响因子：
3.5
通讯作者：
Meyuhas, O
中科院分区：
文献类型：
--
作者：
Biberman, Y;Meyuhas, O
脊椎动物TOP mRNA含有5'末端寡嘧啶段（5' TOP），其在非生长细胞或无细胞翻译系统中受到选择性翻译抑制。在本研究中，我们在体外监测了代表驼鹿核糖体蛋白S16 mRNA 5'末端的16个核苷酸长的寡核苷酸对TOP和非TOP mRNA翻译的影响，我们的结果表明，温和型序列（但不是其突变体对应物）去抑制含有5 ′ TOP基序的mRNA的翻译，但不能刺激非TOP mRNA的翻译，即使后者与其含有5 ′ TOP的对应物仅相差一个核苷酸，用小麦胚芽提取物和兔网织红细胞裂解物也获得了类似的结果。因此，TOP mRNA的翻译抑制似乎是通过可滴定的阻遏物的积累而不是通过激活物的丢失在体外实现的，并且该阻遏物识别具有不同的5'TOP基序组的多个TOP mRNA。(C)1999年欧洲生物化学学会联合会。
Vertebrate TOP mRNAs contain a 5' terminal oligopyrimidine tract (5' TOP), which is subject to selective translational repression in non-growing cells or in cell-free translation systems. In the present study, we monitored in vitro the effect of increasing amounts of a 16 nucleotides long oligoribonucleotide representing the 5' terminus of moose ribosomal protein S16 mRNA on the translation of TOP and non-TOP mRNAs, Our results demonstrate that the mild-type sequence (but not its mutant counterparts) derepresses the translation of mRNAs containing 5' TOP motifs, but failed to stimulate the translation of non-TOP mRNAs, even if the latter differed only by a single nucleotide from their 5' TOP-containing counterparts, Similar results have been obtained with both wheat germ extract and rabbit reticulocyte lysate. It appears, therefore, that translational repression of TOP mRNAs is achieved in vitro by the accumulation of a titratable repressor rather than by the loss of an activator and that this repressor recognizes multiple TOP mRNAs,vith a diverse set of 5' TOP motifs. (C) 1999 Federation of European Biochemical Societies.