Extremely high inhibition activity of photoluminescent carbon nanodots toward cancer cells
Extremely high inhibition activity of photoluminescent carbon nanodots toward cancer cells
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DOI：
10.1039/c3tb00545c
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Chang, Huan-Tsung
中科院分区：
文献类型：
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作者：
Hsu, Pin-Che;Chen, Po-Cheng;Chang, Huan-Tsung
从使用过的绿茶中通过研磨、煅烧和离心得到平均直径为3.4+/-0.8nm、量子产率为4.3%的高水溶性、生物相容性和光致发光的碳纳米点(C-dot)。所制备的碳点在高离子强度介质（例如 500 mM NaCl）和光照射下稳定，允许记录 MCF-10A、MCF-7 和 MDA-MB-231 细胞的图像。碳点主要位于细胞膜和细胞质中，有激发波长细胞图像的证据。相对于儿茶素，碳点对MCF-7和MDA-MB-231癌细胞的生长具有更高的抑制效率，而对MCF-10A正常细胞的毒性更低。碳点的抑制活性与大量活性氧的产生有关。血浆样品的凝血酶原时间 (PT) 测定显示碳点具有出色的生物相容性。据我们所知，碳点首次对 MCF-7 和 MDA-MB-231 癌细胞分别提供高达 80% 和 82% 的抑制效率，显示出其作为癌症抑制剂的巨大潜力。
Highly water-soluble, biocompatible, and photoluminescent carbon nanodots (C-dots) having an average diameter 3.4 +/- 0.8 nm and a quantum yield 4.3% are obtained from used green tea through grinding, calcination and centrifugation. The as-prepared C-dots are stable in high-ionic-strength media (e.g. 500 mM NaCl) and under light irradiation, allowing images of MCF-10A, MCF-7 and MDA-MB-231 cells to be recorded. The C-dots are mostly localized in the cell membranes and cytoplasms, with evidence of excitation-wavelength cell images. Relative to catechin, the C-dots provide greater inhibition efficiency of the growth of MCF-7 and MDA-MB-231 cancer cells, with lower toxicity for the MCF-10A normal cells. The inhibitory activity of C-dots is associated with the generation of greater amounts of reactive oxygen species. A prothrombin time (PT) assay of plasma samples reveals excellent biocompatibility of the C-dots. To the best of our knowledge, for the first time the C-dots provide inhibition efficiencies up to 80% and 82% for MCF-7 and MDA-MB-231 cancer cells, respectively, showing their high potential as cancer inhibitors.