Three-dimensional biomaterials for the study of human pluripotent stem cells
Three-dimensional biomaterials for the study of human pluripotent stem cells
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DOI：
10.1038/nmeth.1671
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发表时间：
2011-09-01
期刊：
影响因子：
48
通讯作者：
Ferreira, Lino S.
中科院分区：
文献类型：
--
作者：
Kraehenbuehl, Thomas P.;Langer, Robert;Ferreira, Lino S.
人多能干细胞（hPSC）的自我更新和分化通常在平坦的二维（2D）环境中进行研究。在这个角度来看，我们认为，3D模型系统可能需要另外，因为他们模仿自然的3D组织组织更密切。我们调查了使用3D生物材料扩增未分化hPSC、定向分化hPSC和体内移植分化hPSC的方法。
The self-renewal and differentiation of human pluripotent stem cells (hPSCs) have typically been studied in flat, two-dimensional (2D) environments. In this Perspective, we argue that 3D model systems may be needed in addition, as they mimic the natural 3D tissue organization more closely. We survey methods that have used 3D biomaterials for expansion of undifferentiated hPSCs, directed differentiation of hPSCs and transplantation of differentiated hPSCs in vivo.