A general ribonuclease assay using methylene blue
A general ribonuclease assay using methylene blue
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DOI：
10.1006/abio.1996.0326
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发表时间：
1996-08-15
影响因子：
2.9
通讯作者：
Hahn, U
中科院分区：
文献类型：
--
作者：
GreinerStoeffele, T;Grunow, M;Hahn, U
核糖核酸酶（RNase）的活性已通过监测亚甲蓝嵌入高分子量RNA后最大吸光度的变化来测定。酵母RNA与亚甲基蓝预孵育后，可在688 nm波长下用荧光光度法跟踪初始水解速率。这允许用其可能的天然底物RNA代替目前使用的人工底物，如二核苷磷酸或环状单磷酸，对RNA酶进行酶动力学研究。(C)出版社：Academic Press，Inc.
Ribonuclease (RNase) activity has been assayed by monitoring the shift in the absorbance maximum of methylene blue upon intercalation into high-molecular-weight RNA. After preincubation of yeast RNA with methylene blue, the initial rate of hydrolysis can be followed spectrophotometrically at a wavelength of 688 nm. This allows enzyme kinetic studies of RNases with their probable native substrate, RNA, in place of the artificial ones, such as dinucleoside phosphates or cyclic monophosphates, currently used. (C) 1996 Academic Press, Inc.