Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius.
Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius.
复制标题
DOI：
10.1099/ijs.0.65192-0
复制
发表时间：
2007-12
影响因子：
2.8
通讯作者：
M. Trujillo;R. Kroppenstedt;Carmen Fernández-Molinero;P. Schumann;E. Martínez-Molina
中科院分区：
文献类型：
--
作者：
M. Trujillo;R. Kroppenstedt;Carmen Fernández-Molinero;P. Schumann;E. Martínez-Molina
进行了一项研究以确定从羽扇豆根瘤中分离出的六种放线菌菌株的分类地位。该菌株为丝状、革兰氏阳性并在菌丝顶端产生单孢子。系统发育、化学分类学和形态学分析表明，所有六株菌株均属于小单孢菌属。根据16S rRNA基因序列数据，将菌株分为与Mirobrigensis小单孢菌、松本小单孢菌和紫色小单孢菌中度相关的两个簇。脂肪酸模式也支持菌株的划分，两组之间的 iso-15 : 0、iso-16 : 0、iso-16 : 1 和 iso-17 : 0 含量存在显着差异。此外，两组还表现出生理差异，包括阿拉伯糖、海藻糖、丙氨酸和蔗糖的利用以及木聚糖水解。最后，DNA-DNA 杂交和核糖分型研究证实，每个群体都代表一个新物种。根据基因型和表型数据，新物种羽扇豆小单孢菌 sp.十一月（类型菌株 Lupac 14N(T) =DSM 44874(T) =LMG 24055(T)）和 Micromonospora saelicesensis sp.十一月（类型菌株 Lupac 09(T) =DSM 44871(T) =LMG 24056(T)）被建议。
A study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius. The strains were filamentous, Gram-positive and produced single spores at the tip of the hyphae. Phylogenetic, chemotaxonomic and morphological analyses demonstrated that all six strains belonged to the genus Micromonospora. According to the 16S rRNA gene sequence data, the strains were divided into two clusters that are moderately related to Micromonospora mirobrigensis, Micromonospora matsumotoense and Micromonospora purpureochromogenes. Fatty acid patterns also supported the division of the strains, and significant differences between the two groups were found in the amounts of iso-15 : 0, iso-16 : 0, iso-16 : 1 and iso-17 : 0. Furthermore, the two groups showed physiological differences which included utilization of arabinose, trehalose, alanine and sucrose and xylan hydrolysis. Finally, DNA-DNA hybridization and ribotyping studies confirmed that each group represents a novel species. Based on the genotypic and phenotypic data, the novel species Micromonospora lupini sp. nov. (type strain Lupac 14N(T) =DSM 44874(T) =LMG 24055(T)) and Micromonospora saelicesensis sp. nov. (type strain Lupac 09(T) =DSM 44871(T) =LMG 24056(T)) are proposed.