Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice.
Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice.
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DOI：
10.1038/sj.bjc.6690742
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发表时间：
1999-10
影响因子：
8.8
通讯作者：
Tahara E
中科院分区：
文献类型：
--
作者：
Kitadai Y;Takahashi Y;Haruma K;Naka K;Sumii K;Yokozaki H;Yasui W;Mukaida N;Ohmoto Y;Kajiyama G;Fidler IJ;Tahara E
肿瘤细胞的生长和扩散依赖于充足的血管系统。我们以前曾报道过白细胞介素-8（IL-8）的表达与胃癌的血管生成直接相关。为了证实IL-8在人胃癌血管生成和致瘤性中的作用，我们采用脂质体法，用IL-8表达载体或对照载体稳定转染人TMK-1胃癌细胞（低内源性IL-8）。用IL-8转染不影响培养细胞的增殖，但转染细胞的培养上清液（而不是对照）刺激人脐静脉内皮细胞的增殖。将IL-8转染的细胞和对照细胞注射到裸鼠的胃壁中。与对照细胞相比，IL-8转染的细胞产生快速生长的高度血管化的肿瘤。这些结果为IL-8在人胃癌血管生成和致瘤性中的作用提供了直接证据。© 1999癌症研究运动
The growth and spread of tumour cells depends on adequate vasculature. We have previously reported that the expression of interleukin-8 (IL-8) directly correlates with the vascularity of human gastric carcinomas. To provide evidence for a causal role of IL-8 in angiogenesis and tumorigenicity of human gastric cancer, we used the lipofectin method to stably transfect the human TMK-1 gastric carcinoma cells (low endogenous IL-8) with an IL-8 expression vector or control vector. Transfection with IL-8 did not affect the proliferation of cultured cells, yet the culture supernatants of the transfected (but not control) cells stimulated proliferation of human umbilical vein endothelial cells. The IL-8-transfected and control cells were injected into the gastric wall of nude mice. IL-8-transfected cells produced rapidly growing, highly vascular neoplasms as compared to control cells. These results provide direct evidence for the role of IL-8 in the angiogenesis and tumorigenicity of human gastric carcinomas. © 1999 Cancer Research Campaign