Parallels between cytokinesis and retroviral budding: A role for the ESCRT machinery
Parallels between cytokinesis and retroviral budding: A role for the ESCRT machinery
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DOI：
10.1126/science.1143422
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发表时间：
2007-06-29
期刊：
影响因子：
56.9
通讯作者：
Martin-Serrano, Juan
中科院分区：
文献类型：
--
作者：
Carlton, Jez G.;Martin-Serrano, Juan
在胞质分裂过程中，当分裂的动物细胞分裂成两个子细胞时，最后一个阶段，称为分裂，需要中间体的断裂，一个连接子细胞的薄膜柄。这种膜分裂事件在拓扑学上类似于病毒（如HIV-1）从受感染细胞中出芽。我们发现，两种蛋白参与HIV-1芽殖肿瘤易感基因101（Tsg 101），一个亚基的内体分选复合物所需的运输I（ESCRT-I），和阿利克斯，ESCRT相关蛋白-被招募到中间体在胞质分裂过程中的相互作用与中心体蛋白55（Cep 55），一个中心体和中间体蛋白必需的分裂。Tsg 101、阿利克斯和可能的ESCRT-I的其他组分是完成胞质分裂所必需的。因此，HIV-1出芽和胞质分裂使用相似的细胞组分子集来进行拓扑相似的膜分裂事件。
During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells. This membrane fission event topologically resembles the budding of viruses, such as HIV-1, from infected cells. We found that two proteins involved in HIV-1 budding-tumor susceptibility gene 101 (Tsg101), a subunit of the endosomal sorting complex required for transport I (ESCRT-I), and Alix, an ESCRT-associated protein-were recruited to the midbody during cytokinesis by interaction with centrosome protein 55 (Cep55), a centrosome and midbody protein essential for abscission. Tsg101, Alix, and possibly other components of ESCRT-I were required for the completion of cytokinesis. Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events.