Afferent induction of olfactory glomeruli requires N-cadherin
Afferent induction of olfactory glomeruli requires N-cadherin
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DOI：
10.1016/s0896-6273(04)00158-8
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发表时间：
2004-04-08
期刊：
影响因子：
16.2
通讯作者：
Zipursky, L
中科院分区：
文献类型：
--
作者：
Hummel, T;Zipursky, L
果蝇嗅觉受体神经元（ORN）在触角叶（AL）中精确地表达了外部嗅觉世界，这一结构类似于脊椎动物的嗅球。表达相同气味受体的ORN支配AL（肾小球）内高度组织化的神经纤毛结构中的共同靶标。在正常发育过程中，ORN靶向AL的特定区域，并在与靶树突广泛混合以形成成熟肾小球之前分离成称为原肾小球的亚类特异性聚集体。使用一组ORN亚类特异性标记，我们证明，在成人AL，N-钙粘蛋白（N-cad）突变ORN终端保持隔离从树突的靶神经元。N-cad在肾小球形成中起着至关重要的作用，但在很大程度上是必要的针对适当的区域的AL。我们建议，N-cad，一种嗜同性细胞粘附分子，在一个允许的方式，以促进亚类特异性排序的ORN轴突终末到原肾小球。
Drosophila olfactory receptor neurons (ORNs) elaborate a precise internal representation of the external olfactory world in the antennal lobe (AL), a structure analagous to the vertebrate olfactory bulb. ORNs expressing the same odorant receptor innervate common targets in a highly organized neuropilar structure inside the AL, the glomerulus. During normal development, ORNs target to specific regions of the AL and segregate into subclass-specific aggregates called protoglomeruli prior to extensive intermingling with target dendrites to form mature glomeruli. Using a panel of ORN subclass-specific markers, we demonstrate that in the adult AL, N-cadherin (N-cad) mutant ORN terminals remain segregated from dendrites of target neurons. N-cad plays a crucial role in protoglomerulus formation but is largely dispensible for targeting to the appropriate region of the AL. We propose that N-cad, a homophilic cell adhesion molecule, acts in a permissive fashion to promote subclass-specific sorting of ORN axon terminals into protoglomeruli.