Targeting neural circuitry in zebrafish using GAL4 enhancer trapping
Targeting neural circuitry in zebrafish using GAL4 enhancer trapping
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DOI：
10.1038/nmeth1033
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发表时间：
2007-04-01
期刊：
影响因子：
48
通讯作者：
Baier, Herwig
中科院分区：
文献类型：
--
作者：
Scott, Ethan K.;Mason, Lindsay;Baier, Herwig
我们提出了一个试点增强子陷阱屏幕使用GAL4驱动上游激活序列（UAS）连接的转基因的表达模式决定的内源性增强子在斑马鱼的表达。所呈现的模式包括在整个幼虫脑中的神经元的小子集中的表达，在某些情况下，其持续到成年。通过UAS驱动的Kaede的靶向光转换和UAS驱动的GFP在单细胞中的多样化表达，我们开始表征标记电路的细胞组分。
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.